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目的：检验氧化损伤的牛RPE细胞是否存在p53基因表达的变化和人参皂甙Rg1、Rb1和维生素E对其的影响，以期进一步研究RPE细胞氧化损害的机理.方法：建立牛RPE细胞氧化损伤模型，原位杂交技术测定RPE细胞p53mRNA的表达，免疫组化技术测定p53蛋白表达.结果：p53蛋白表达阳性的牛RPE细胞的细胞核染成黄色，与次黄嘌吟／黄嘌吟氧化酶（HX／XO）组p53蛋白阳性的细胞数相比较，正常对照组，Rg1（0.1mg.L-1）组及VitE（10mg.L-1）组存在显著性差异（P＜0.05），而与Rb1（10mg.L-1）组无显著性差异.原位杂交法测定P53mRNA表达，牛RPE细胞P53mRNA表达阳性可在细胞浆内见到位于细胞周边部的蓝紫色絮状物，分别与HX／XO组阳性的细胞相比较，正常对照组，Rg1组及VitE组存在显著性差异（P＜0.05），而与Rb1组无显著性差异.结论：氧化损伤可导致基因P53mRNA及其蛋白在RPE细胞内的表达明显增加.人参皂甙Rg1和VitE对抗氧自由基对RPE细胞损伤的机制可能是通过清除氧自由基，影响凋亡基困p53的表达，从而有效地抑制氧自由基对RPE细胞的损伤.
OBJECTIVE: To investigate the effect of oxidative damage of RPE cells on the oxidative damage of RPE cells.METHODS: The oxidative damage of RPE cells induced by oxidative stress was investigated by detecting the expression of p53 gene and the effects of ginsenoside Rg1, Rb1 and vitamin E.Methods: The expression of p53 mRNA in RPE cells was detected by in situ hybridization and the expression of p53 protein was detected by immunohistochemistry.Results: The nuclei of cattle RPE cells with positive p53 protein were stained yellow, which was similar to hypoxanthine / xanthine oxidase (HX / (P <0.05), but not with the group of Rb1 (0.1 mg.L-1) and VitE (10 mg.L-1) 10mg.L-1) .The P53mRNA expression was detected by in situ hybridization and the positive expression of P53mRNA in bovine RPE cells could be seen in the cytoplasm of the cells in the peripheral part of the blue-purple floc, respectively, with the HX / XO group (P <0.05), but no significant difference with Rb1 group.Conclusion: Oxidative damage can lead to the expression of P53 mRNA and protein in RPE cells expression Significantly increased. VitE ginsenoside Rg1 and oxygen free radical mechanism of RPE cells may be damaged by scavenging oxygen free radicals, expression of p53 apoptotic group trapped, thereby effectively suppressing oxygen free radical damage to RPE cells.