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目的 研究S 甲基异硫脲 (SMT)对梗塞兔心肌组织内诱导型一氧化氮合酶 (iNOS)活性的抑制作用。方法 对左房心耳和心尖中间弯曲的冠状动脉前外侧枝进行结扎 ,建立心肌梗塞模型。应用L 精氨酸转换为L 胍氨酸法测定家兔心肌梗塞区和非梗塞区诱导型一氧化氮合酶的活性 ,同时观察S 甲基异硫脲和NW 硝基 L 精氨酸 (L NNA)对诱导型一氧化氮合酶活性的抑制作用。结果 冠状动脉结扎后 48h、72h和 96h ,心肌梗塞区诱导型一氧化氮合酶活性比非手术组显著增加 (P <0 0 1) ,并在 72h达到峰值 ;梗塞后 72h左室壁cGMP含量也明显增加 (P <0 0 1) ;同时SMT能明显降低梗塞区心肌诱导型一氧化氮合酶的活性 (P<0 0 1)。结论 SMT能明显抑制梗塞兔心肌iNOS的活性 ,提示SMT对改善心脏功能和治疗急性心肌梗塞具有一定的临床效果。
Objective To investigate the inhibitory effect of S-methylisothiourea (SMT) on inducible nitric oxide synthase (iNOS) activity in infarcted rabbit myocardium. Methods The left atrial appendage and the apex of the middle curved anterior coronary artery were ligated to establish a myocardial infarction model. The activity of inducible nitric oxide synthase in myocardial infarction area and non-infarcted area of rabbits was measured by L-arginine conversion to L-citrulline method. Meanwhile, the activities of S-methylisothiourea and NW nitro L-arginine (L NNA) on the inducible nitric oxide synthase activity. Results At 48h, 72h and 96h after coronary artery ligation, the activity of inducible nitric oxide synthase in myocardial infarction area was significantly higher than that of non-operation group (P <0.01) and peaked at 72h. The content of cGMP in left ventricular wall 72h after infarction (P <0.01). At the same time, SMT could obviously decrease the activity of inducible nitric oxide synthase in infarcted area (P <0.01). Conclusion SMT can significantly inhibit the activity of iNOS in infarcted rabbit myocardium, suggesting that SMT has some clinical effects on improving cardiac function and treating acute myocardial infarction.