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目的 通过检测染色体结构异常患者的精子染色体结构 ,分析其减数分裂中染色体分离规律。方法 用荧光原位杂交方法 ,其中例 1核型为 45 ,XY,ter rea(15 ;18) (p13∶ p11) ,用 CEP15 ,CEP18探针 ,例 2核型为 45 ,XY,(13;14) ,用 Vysis L SI 13q14,Tel Vysion14q探针 ,分别对他们的精子进行荧光原位杂交检测。结果 例 1的 2 2 14条精子中 ,15 / 18占 5 8.4% ,15 ,15 / 18占 5 .2 8% ,- / 18占 6 .86 % ,15 / 18,18占 7.77% ,15 / - -占 7.6 8%。例 2的 15 2 4条精子中 ,13q/ 14q占 49.6 7% ,13q(13q,13q/ 14q)或 14q(13q/14q,14q)分别占 8.86 %、8.2 7% ,13q/ - -占 7.6 8% ,- - / 14q占 5 .18%。结论 通过荧光原位杂交方法检测染色体结构异常患者的精子 ,可以分析其减数分裂中染色体分离规律。
Objective To detect the chromosome segregation in meiosis by detecting the chromosome structure of sperm in patients with chromosomal abnormalities. Methods The fluorescence in situ hybridization was used. The karyotype of case 1 was 45, XY, ter rea (15; 18) (p13: p11) 14), respectively, with Vysis L SI 13q14, Tel Vysion14q probe, respectively, their sperm fluorescence in situ hybridization detection. Results In the 2 2 14 sperm samples in Example 1, 15/18 accounted for 5 8.4%, 15/15% accounted for 5.28%, - / 18 accounted for 6.86%, 15/18, 18 7.77%, 15 / - - 7.6 8%. Of 15 2 4 spermatozoa in Example 2, 13q / 14q accounted for 49.6 7%, 13q (13q, 13q / 14q) or 14q (13q / 14q, 14q) accounted for 8.86%, 8.27% and 13q / 8%, - - / 14q accounted for 5.18%. Conclusion The fluorescence in situ hybridization detection of chromosome abnormalities in patients with sperm, meiosis chromosome segregation can be analyzed.