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采用反相高效液相色谱梯度洗脱法 ,用ODS柱 ,以甲醇 醋酸 三乙胺 水为流动相 ,在 2 30nm处同时测定唐威胶囊中盐酸小檗碱及葛根素的含量。平均添加回收率 (n =5)和RSD值分别为 98 2 %和 1 6%(盐酸小檗碱 ) ;97 5%和 1 8%(葛根素 )。
The contents of berberine hydrochloride and puerarin in Tang Wei capsule were determined simultaneously at 2 30 nm by reversed-phase high performance liquid chromatography with gradient elution and ODS column with methanol-methanol-triethylamine as mobile phase. The average recoveries (n = 5) and RSD values were 98 2% and 16% (berberine hydrochloride); 97.5% and 18% (puerarin), respectively.