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目的研究重组人乳铁蛋白(recombinant human lactoferrin,rHLF)分别在体外模拟胃液和模拟肠液中的消化稳定性。方法采用模拟胃液和模拟肠液在体外建立模拟胃肠环境消化体系,测定重组人乳铁蛋白在胃肠环境中的稳定性。重组人乳铁蛋白在模拟胃、肠液中的浓度分别为5.0和2.0 mg/ml。在重组人乳铁蛋白与模拟胃/肠液反应后的0、15、30 s和1、2、5、10、20、30、60 min取样,根据SDS-PAGE凝胶电泳结果,判断重组人乳铁蛋白在模拟胃、肠液环境中的稳定性。结果重组人乳铁蛋白在体外模拟胃液中2 min内降解,在模拟肠液中60 min内不能完全降解。结论重组人乳铁蛋白在体外模拟人体胃肠环境中易被降解消化。
Objective To study the digestive stability of recombinant human lactoferrin (rHLF) in vitro and in simulated intestinal fluid. Methods Simulated gastric juice and simulated intestinal fluid were used to establish a gastrointestinal digestive system in vitro and the stability of recombinant human lactoferrin in gastrointestinal environment was determined. Recombinant human lactoferrin in simulated gastric and intestinal fluid concentrations were 5.0 and 2.0 mg / ml, respectively. Samples were taken at 0, 15, 30 s and 1, 2, 5, 10, 20, 30 and 60 min after reaction of recombinant human lactoferrin with simulated gastric / intestinal fluid. According to the results of SDS-PAGE gel electrophoresis, recombinant human milk Ferritin in the simulated gastric and intestinal fluid stability. Results Recombinant human lactoferrin degraded in vitro in simulated gastric juice for 2 min and could not be completely degraded in simulated intestinal fluid within 60 min. Conclusions Recombinant human lactoferrin is easily degraded and digested in the simulated human gastrointestinal environment in vitro.