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用农杆菌介导玉米愈伤组织的转化,其筛选的结果得到的抗性愈伤组织受玉米愈伤组织的继代时 间、浸染的农杆菌菌液浓度、共培养的温度以及其共培养时间等因素的影响。玉米愈伤继代后7～9d,农杆菌 浓度为OD600值0.3左右、共培养温度约22℃、培养时间3d时,抗性愈伤的获得率最高。
Agrobacterium-mediated transformation of maize callus, the selection of the results obtained by the resistance of callus by the callus of the corn subculture time, the concentration of Agrobacterium rhizobacteria, co-culture temperature and co-culture time And other factors. After the callus was subcultured for 7-9 days, the Agrobacterium concentration was about 0.3 at OD600, and the co-culture temperature was about 22 ℃. The highest yield of resistant callus was obtained at 3 days.