基于黄瓜基因组重测序结果,搜索InDel位点,按照每隔1～3M个碱基对的距离选择并设计遍布全基因组的代表性InDel引物134对,以16份黄瓜典型种质检测其有效性。结果显示,134对引物均获得扩增产物,其中具有多态性的引物116对,占引物总数的86.6%。116对引物充分揭示出16份种质的多样性和特异性。本研究所开发的InDel分子标记将为黄瓜种质资源和分子遗传育种研究提供有力的遗传工具。 Based on the results of cucumber genome resequencing, InDel locus was screened, 134 pairs of representative InDel primers were selected and designed to spread over the whole genome every 1 ~ 3 M base pairs, and 16 cucumber typical germplasms were tested for their effectiveness. The results showed that 134 pairs of primers obtained amplification products, of which 116 pairs of polymorphic primers, accounting for 86.6% of the total number of primers. 116 pairs of primers fully revealed the diversity and specificity of 16 germplasm. InDel molecular markers developed in this study will provide powerful genetic tools for the research of cucumber germplasm resources and molecular genetics and breeding.