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鉴定比较供体亲本京411和轮回亲本晋麦47及其34个近等基因系材料之间产量和水分利用效率(WUE),同时测定不同生育期的气冠温差(CTD),分析WUE在这些材料之间的变异范围,筛选WUE与轮回亲本显著差异的材料用于QTL定位,同时探索干旱条件下CTD与产量和WUE的关系及其随生育期进程变化的趋势。利用防雨棚和渗漏池开展模拟干旱试验。结果表明,34个近等基因系及其父母本WUE为1.30~1.92 kg/m~3,其中9个品系WUE显著低于轮回亲本晋麦47,表明WUE为多基因控制的数量性状,而且存在明显的加性效应。CTD与产量和WUE均呈极显著正相关关系。不同的是,随生育期进程推进,CTD与产量的相关性增强,在灌浆期最高,R2达到0.684 9,而与WUE的相关性减弱,R2在拔节-孕穗期最高,达到0.769 8。研究初步表明,CTD可以作为干旱条件下产量和WUE的鉴定指标。
Identification and comparison of yield and water use efficiency (WUE) between the donor parent Jing 411 and the recurrent parent Jinmai 47 and its 34 near-isogenic lines, as well as the determination of the canopy temperature difference (CTD) at different growth stages and the analysis of WUE in these The range of variation between the two materials was used to screen QTL for the significant difference between WUE and recurrent parents. The relationship between CTD and yield and WUE under drought stress and the trend of its changes with the growing period were also explored. Simulated drought test using rain shelter and leaking tank. The results showed that the WUE of 34 near-isogenic lines and their parents were 1.30-1.92 kg / m ~ 3, of which 9 were lower than that of the recurrent parent Jinmai 47, suggesting that WUE is a quantitative trait controlled by polygene, Obvious additive effect. There was a significant positive correlation between CTD and yield and WUE. The difference is that with the progress of the growth period, the correlation between CTD and yield is enhanced. In the grain filling stage, the R2 reached 0.684 9, but decreased with WUE. R2 reached the highest at jointing-booting stage, reaching 0.769 8. Preliminary studies have shown that CTD can be used as indicators of drought yield and WUE identification.