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目的:建立巨噬细胞和BALB/c小鼠的脂多糖(LPS)脓毒症模型和GTS-21胆碱能抗炎通路模型。方法:RAW264.7和BALB/c小鼠用于观察不同剂量下LPS制作的脓毒症模型和GTS-21制作的胆碱能抗炎通路(CAP)模型。通过酶联免疫吸附试验(ELISA)检测,探索使细胞和小鼠存活时间大于24h,且肿瘤坏死因子(TNFα)和高迁移率族蛋白B1(HMGB1)达最高浓度时的LPS剂量。在此实验基础上寻找有效降低TNFα和HMGB1的最佳GTS-21剂量。结果:RAW264.7细胞模型构建时,脓毒症模型中TNFα和HMGB1表达最高的LPS剂量为50mg/ml,胆碱能抗炎通路模型中最佳GTS-21剂量6μg/ml。小鼠脓毒症模型中,LPS剂量为15mg/kg时,TNFα和HMGB1表达最高且小鼠存活>24h,小鼠CAP模型最佳GTS-21剂量4mg/kg。结论:得出LPS小鼠脓毒症模型和GTS-21胆碱能抗炎通路模型的最佳用药剂量,为探索脓毒症和胆碱能抗炎通路的实验研究奠定基础。
AIM: To establish a model of lipopolysaccharide (LPS) sepsis and GTS-21 cholinergic anti-inflammatory pathway in macrophages and BALB / c mice. Methods: RAW264.7 and BALB / c mice were used to observe the sepsis model produced by LPS at different doses and the cholinergic anti-inflammatory pathway (CAP) model made by GTS-21. The LPS dose at the highest concentration of tumor necrosis factor (TNFα) and high mobility group box 1 (HMGB1) was explored by enzyme-linked immunosorbent assay (ELISA). Based on this experiment, we found the best dose of GTS-21 that can effectively reduce TNFα and HMGB1. Results: When the RAW264.7 cell model was constructed, the LPS dose with the highest TNFα and HMGB1 expression in sepsis model was 50mg / ml and the best GTS-21 dose in cholinergic anti-inflammatory pathway model was 6μg / ml. Mouse sepsis model, LPS dose of 15mg / kg, the highest TNFα and HMGB1 expression and survival of mice> 24h, the best mouse model CAP GTS-21 dose of 4mg / kg. Conclusion: The best dose of LPS mouse sepsis model and GTS-21 cholinergic anti-inflammatory pathway model is obtained, which lays the foundation for the experimental study of sepsis and cholinergic anti-inflammatory pathways.