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通过盆栽试验探究外源喷施油菜素内酯(EBR)对开花期大豆高温胁迫下抗逆性的影响。试验于2014-2015年在南京农业大学牌楼试验基地进行,以大豆品种南农99-6为试验材料,在花后7 d进行EBR预处理,花后11 d进行高温胁迫,持续4 d。分别在花后11 d(EBR处理结束)、15 d(高温胁迫结束)、19 d(恢复常温后4 d)、27 d(恢复常温后8 d)取样。探究外源EBR对大豆常温及高温胁迫下光合、生物量及产量品质的影响。结果表明:外源EBR显著提高了常温和高温胁迫大豆叶片净光合速率和干物质积累量,且增加了百粒重和单株产量。除此之外,外源EBR显著提高了籽粒中的蛋白质含量、可溶性总糖及淀粉的含量,降低了粗油脂的含量,改善了大豆籽粒的品质。综上所述,外源喷施EBR预处理不但可以通过提高常温和高温条件下大豆叶片的光合能力,促进干物质积累,提高产量,而且提高了籽粒中蛋白质含量,改善了籽粒品质。
Pot experiments were conducted to investigate the effects of exogenous brassinosteroid (EBR) on the stress resistance of flowering soybean under high temperature stress. The experiment was carried out in the arch test base of Nanjing Agricultural University from 2014 to 2015. The soybean variety Nannong 99-6 was used as experimental materials and pretreated with EBR for 7 days and then subjected to high temperature stress for 11 days after flowering for 4 days. The samples were taken after 11 days of anthesis (end of EBR treatment), 15 days (end of high temperature stress), 19 days (4 days after normal temperature resumed) and 27 days (8 days after normal temperature resumed). Effects of exogenous EBR on photosynthesis, biomass and yield and quality of soybean under normal and high temperature stress. The results showed that exogenous EBR significantly increased the net photosynthetic rate and dry matter accumulation of soybean leaves under normal and high temperature stress, and increased the 100-grain weight and yield per plant. In addition, the exogenous EBR significantly increased the protein content, soluble total sugar and starch content in the grain, reduced the crude fat content and improved the quality of the soybean grain. In summary, EBR pretreatment not only promoted photosynthetic ability of soybean leaves at room temperature and high temperature, promoted the accumulation of dry matter and increased the yield, but also increased the protein content in grain and improved the grain quality.