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人乳头瘤病毒(简称HPV)能引起人皮肤及粘膜的多种良恶性肿瘤,特别多见于女性生殖系统如宫颈癌、尖锐湿疣和假性湿疣。但由于HPV尚不能在体外细胞培养中增殖,因此目前对其研究主要用核酸杂交和PCR方法,检测HPV DNA。本文采用光敏生物素标记HPV16、HPV18 DNA探针,分别对宫颈癌组织进行斑点杂交,检测宫颈癌组织中HPV16、18DNA并以正常宫颈组织做对照。以同样方法用HPV6b DNA探针检测尖锐湿疣及假性湿疣组织中HPV6b DNA。结果如下:18例宫颈癌组织中仅有1例与HPV16 DNA探针杂交阳性(5.7%);10例与HPV18杂交刚性(55.6%);4例为HPV16+18混合杂交阳性(22.2%)。12例尖锐湿疣组织中8例与HPV6b DNA杂交阳性(66.7%);12例假性湿疣组织中有6例与HPV6b探针杂交阳性(50%)。10例正常宫颈组织对照均为阴性。
Human papillomavirus (referred to as HPV) can cause a variety of benign and malignant tumors of human skin and mucous membranes, especially in the female genital system such as cervical cancer, genital warts and pseudoknot. However, since HPV can not yet proliferate in vitro in cell culture, HPV DNA is currently detected by nucleic acid hybridization and PCR. In this paper, biotin-labeled biotin HPV16, HPV18 DNA probe, respectively, dot blot hybridization to cervical cancer, detection of cervical cancer tissue HPV16,18 DNA and normal cervical tissue as a control. HPV6b DNA probe was used to detect HPV6b DNA in condyloma acuminatum and condyloma acuminata in the same way. The results were as follows: only 1 of 18 cervical cancer tissues was positive for HPV16 DNA probe (5.7%); 10 had hybridization with HPV18 (55.6%); 4 had HPV16 + 18 positive hybridization (22.2%). Eight of 12 condyloma acuminatum specimens were positive for HPV6b DNA hybridization (66.7%). Six of 12 cases of pseudocondense were positive for HPV6b probe hybridization (50%). Ten cases of normal cervical tissue control were negative.