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目前认为大多数的免疫异常都是由于成熟的T细胞亚群变动所致,免疫调节功能紊乱在肿瘤发病中有重要作用.T细胞亚群的检测可部分了解肿瘤发病的机理和病理免疫反应是否还在继续进行,以达到有助于诊断和治疗的目的.本文对淋巴瘤患者外周血中T淋巴细胞亚群的改变报告如下.1 对象和方法1.1 对象 正常对照组30例,男20例,女10例,平均年龄35.5(22～51)岁,均为献血员.淋巴结结核15例男6例,女9例,平均年龄28.3(11～52)岁,肿大淋巴结均经细胞学检查,病程呈良性经过.淋巴瘤患者14例,男11例,女3例,平均年龄47.9(19～77)岁,均经病检证实,非何杰金淋巴瘤13例,Ⅲ Ⅳ期患者9例.1.2 方法 T淋巴细胞亚群单克隆抗体由武汉生物制品研制所提供.用淋巴细胞分离液制备单个核细胞,用RPMI—1640液调整细胞数5×10~6/ml,取细胞悬液100μl加入培养板中,每孔分别加入T淋巴细胞单克隆抗体50μl(CD_3、CD_4、CD_8)置4℃反应45分钟.洗涤细胞2次后再加入抗人IgG-FITC荧光抗体25μl,4℃反应45分钟后洗涤3次,滴片.在荧光显微镜下,计数200个淋巴细胞中CD_3~+、CD_4~+、CD_8~+细胞并计算其百分率.1.3 统计学处理 用t或t′检验.2 结果正常对照、淋巴结构和淋巴瘤患者外周血T细胞亚群百分数及CD_4/CD_8值见附表.与正常对照组比
It is thought that most of the immune abnormalities are caused by the changes of mature T cell subsets, and the dysfunction of immune regulation plays an important role in the tumorigenesis.The detection of T cell subsets can partly understand the mechanism of tumor pathogenesis and pathological immune response Continue to be carried out in order to achieve the purpose of helping diagnosis and treatment.This article on the changes of T lymphocyte subsets in peripheral blood of patients with lymphoma are reported as follows.1 objects and methods 1.1 object normal control group of 30 patients, 20 males, The average age was 35.5 (ranged from 22 to 51 years), all of whom were blood donors.The lymph node tuberculosis was found in 15 males and 6 females and 9 females, with an average age of 28.3 (ranged from 11 to 52 years). The enlarged lymph nodes were examined by cytology, The course of the disease was benign.The lymphoma patients in 14 cases, 11 males and 3 females, mean age 47.9 (19 to 77) years old, were confirmed by pathological examination, 13 cases of non-Hodgkin’s lymphoma, Ⅲ Ⅳ stage in 9 patients .1.2 Methods Monoclonal antibody of T lymphocyte subsets was provided by Wuhan Institute of Biological Products Preparation of mononuclear cells with lymphocyte separation liquid, the number of cells was adjusted 5 × 10 ~ 6 / ml with RPMI-1640 solution, the cell suspension 100μl Added to the culture plate, each well were added T lymphocyte monoclonal antibody 50μl (CD 3, CD_4, and CD_8) for 45 minutes at 4 ° C. After washing the cells twice, 25 μl of anti-human IgG-FITC fluorescent antibody was added, and the plate was washed three times at 4 ° C for 3 times and then spotted. Under a fluorescence microscope, CD_3 ~ +, CD_4 ~ +, CD_8 ~ + cells in lymphocytes and calculate the percentage.3.3 Statistical analysis using t or t ’test.2 Results The percentage of T cell subsets in peripheral blood of patients with lymphoid structure and lymphoma and The value of CD_4 / CD_8 is shown in the attached table, compared with the normal control group