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目的:探讨不同浓度苦参水提液对细胞增殖的影响。方法:采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度(5、2、1、0.5、0.05、0.025、0.005 mg/mL)的苦参水提物在不同的时间点(24、48、72 h)对HepG-2、L02细胞增殖的影响。10 g/(kg.d)的苦参水提液予ICR小鼠灌胃,10%含药血清培养L02细胞,MTT比色法检测不同的时间点(24、48、72 h)对细胞增殖的影响。以△OD值(各组加药平均值-正常组平均值)表征抑制或促进作用。结果:苦参水提物浓度≤2 mg.mL-1时对细胞增殖有一定促进作用,尤其以0.5、1 mg.mL-1的促进作用最明显(P<0.01),当苦参水提物浓度达到2 mg.mL-1,处理72 h时,细胞增殖出现抑制作用。含药血清能显著促进L02细胞的增殖。结论:苦参水提物在一定浓度范围内促进细胞增殖,高浓度抑制细胞增殖。
Objective: To investigate the effects of different concentrations of Sophora flavescens aqueous extract on cell proliferation. Methods: MTT colorimetry was used to detect the aqueous extract of Sophora flavescens with different concentrations (5,2,1,0.5,0.05,0.025,0.005 mg / mL) at different time points (24 , 48, 72 h) on the proliferation of HepG-2 and L02 cells. The extract of Sophora flavescens (10 g / (kg · d)) was intragastrically administered to ICR mice, and L02 cells were cultured in 10% drug-containing serum. Cell viability was measured by MTT assay at different time points (24, 48 and 72 h) Impact. The effect of inhibition or promotion was characterized by △ OD value (average value of each group plus average of normal group). Results: The extract of Sophora flavescens water extract concentration ≤ 2 mg.mL-1 on cell proliferation has a certain role in promoting, especially 0.5,1 mg.mL-1 the most obvious role in promoting (P <0.01) When the concentration reached 2 mg.mL-1, the cell proliferation was inhibited at 72 h. Drug-containing serum can significantly promote the proliferation of L02 cells. Conclusion: Sophora flavescens aqueous extract can promote cell proliferation in a certain concentration range, and high concentration can inhibit cell proliferation.