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系统阐述了与金纳米粒子(GNPs)放射增敏效应相关实验的方法与结果、影响GNPs放射增敏的因素、GNPs放射增敏的细胞和动物实验表现及其相关机制。同时结合相关实验,分析和比较了15 nm柠檬酸钠包被的GNPs的放射增敏效应,发现GNPs在高LET的碳离子束和低LET的X射线辐照下对Hela细胞的杀伤力随其浓度的增加而增大;在50%的细胞存活率下,当GNPs的质量浓度为7.5μg/m L时,其X射线的剂量减少率和碳离子的相对生物学效应值(RBE)的增加率达到了最大,分别为65.3%和43.6%,同时GNPs共培养细胞24和48 h后,未出现细胞周期同步化的现象。
The methods and results of experiments related to the radiosensitization of gold nanoparticles (GNPs), the factors affecting the radiosensitization of GNPs, the cell and animal performance of GNPs radiosensitizing and the related mechanisms were systematically described. Combined with relevant experiments, the radiosensitization effect of GNPs coated with 15 nm sodium citrate was analyzed and compared. It was found that the lethality of GNPs to Hela cells under the high LET carbon ion beam and low LET X-ray irradiation followed the Concentration of GNPs and the dose-reduction of X-ray and the relative biological effect value (RBE) of carbon ions of GNPs at a concentration of 7.5μg / m L at 50% cell viability The rates reached the maximum at 65.3% and 43.6%, respectively. At the same time, GNPs co-cultured for 24 and 48 h did not show cell cycle synchronization.