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目的:建立小肠急性缺血再灌注损伤模型,确定合适的肠系膜上动脉阻断时间。方法:将70只新西兰兔随机按不同的肠系膜缺血时间(0、15、30、45、60min)分为A、B、C、D、E5组,每组14只,取8只用于恢复血供2h后留取各组兔下腔静脉血标本及肠组织,检测血清中MDA含量的变化,光镜下观察小肠组织形态学变化并对小肠黏膜损伤程度进行评分。另6只用于术后24h、48h及72h生存率的观察。结果:A、B、C组的术后生存率均>83.3%。C、D、E组的MDA含量及肠黏膜损伤评分与A组比较,差异均有显著性(F=12.13～280.24,p<0.01)。结论:肠系膜缺血30min,再灌注2h是建立兔小肠急性再灌注损伤的合适时间。
OBJECTIVE: To establish a model of acute intestinal ischemia-reperfusion injury and determine the appropriate time of superior mesenteric artery occlusion. Methods: Seventy New Zealand rabbits were randomly divided into A, B, C, D and E5 groups according to different time of mesenteric ischemia (0, 15, 30, 45, 60min) After 2 hours of blood supply, the samples of inferior vena cava in each group were collected and the intestinal tissues were collected. The changes of MDA content in serum were detected. The morphological changes of small intestine were observed under light microscope and the damage degree of small intestine was evaluated. The other 6 were used for the observation of survival rate at 24h, 48h and 72h after operation. Results: The postoperative survival rates of group A, B and C were all> 83.3%. Compared with group A, the content of MDA and the score of intestinal mucosal injury in groups C, D and E were significantly different (F = 12.13-280.24, p <0.01). Conclusion: The mesenteric ischemia 30min, reperfusion 2h is the appropriate time to establish rabbit acute reperfusion injury.