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目的观察在内脏高敏感状态下食管酸灌注对中枢神经系统(CNS)各部位Fos蛋白表达的影响,以初步明确CNS参与内脏高敏感性应答和调控的具体部位及中枢传导通路的变化。方法健康SD大鼠36只,随机分为5组。A组：正常对照组6只；B组：0．9%氯化钠溶液对照组7只；C组：食管酸灌注组8只；D组：卵清蛋白(OVA)致敏组7只；E组：OVA+食管酸灌注组8只。采用腹腔注射鸡OVA基础致敏联合食管酸灌注的方法建立食管内脏高敏感性大鼠模型；采用免疫组化方法和显微图像分析等技术研究在生理条件、内脏高敏状态下食管酸灌注后CNS各部位的Fos蛋白激活模式的差异。结果鸡OVA致敏联合食管酸灌注组被激活了一个复杂而广泛大脑网络,其在额顶皮质、岛叶、扣带皮质、中央杏仁核、Kol—liker Fuse核、疑核、臂旁核、下丘脑室旁核、丘脑室旁核、三叉旁核、孤束核、最后区、延髓网状核等核团Fos样免疫活性(FLI)神经元的数目均显著高于其余各组(P＜0．05),但在迷走神经背核、下丘脑视上核、中脑导水管周围灰质、腹外侧眶皮层的FLI神经元数量,与食管酸灌注组比较则无明显改变(P＞O．05)。且该组大鼠的中央杏仁核、臂旁核、室旁核、三叉旁核、孤束核的FLI阳性产物的平均吸光度(A)值亦较其余各组明显增高(P＜0．05)。结论腹腔注射鸡OVA基础致敏对食管酸灌注诱导的CNS内Fos蛋白表达有活化作用,提示内脏高敏感状态可导致CNS不同核团神经元功能发生不同程度的障碍,CNS整合、处理食管感觉传人信息功能异常。
Objective To investigate the effect of esophageal acid perfusion on the expression of Fos protein in various parts of central nervous system (CNS) under visceral hypersensitivity in order to clarify the changes of CNS involved in specific parts of the visceral hypersensitivity response and the central conduction pathways. Methods Thirty-six healthy SD rats were randomly divided into five groups. Group A: normal control group 6; Group B: 0.9% sodium chloride solution control group 7; Group C: esophageal acid perfusion group 8; Group D: ovalbumin (OVA) sensitized group 7; Group E: OVA + esophageal acid perfusion group 8. The rat model of visceral hypersensitivity was established by intraperitoneal injection of OVA basal sensitization combined with esophageal acid perfusion. Immunohistochemistry and microscopic image analysis were used to study the effects of esophageal peroxidation on CNS Differences in Fos protein activation patterns at various sites. Results OVA sensitization combined with esophageal acid perfusion group activated a complex and extensive brain network, with significant changes in the frontal cortex, insula, cingulate cortex, central amygdala, Kol-liker Fuse nucleus, suspicious nucleus, parabrachial nucleus, The number of Fos-like immunoreactive (FLI) neurons in hypothalamic paraventricular nucleus, paravertebral nucleus, paraforlateral nucleus, nucleus tractus solitarius, caudal region and medulla oblongata nucleus were significantly higher than those in other groups (P < 0.05). However, the numbers of FLI neurons in vagal dorsal horn, hypothalamic supraoptic nucleus, midbrain periaqueductal gray and ventral lateral orbital cortex had no significant changes compared with esophageal acid perfusion group (P> 0.05 ). The mean absorbance (A) value of FLI positive products of central amygdala, parabrachial nucleus, paraventricular nucleus, parabrachial nucleus and solitary nucleus of rats in this group were also significantly higher than those in other groups (P <0.05) . Conclusion The basic sensitization of OVA induced by peritoneal inoculation can activate Fos protein expression induced by esophageal acid perfusion in CNS, suggesting that visceral hypersensitivity may lead to different degrees of neuronal dysfunction in CNS. CNS integration and treatment of esophageal sensory transmission Information function is abnormal.