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Lewis~y抗元是结合于Ⅱ型血型寡醣琏的含四的醣结构—Fucl→2Gall→4(Fucl→3)GlcNAC。本实验用四种单克隆抗体,分别识别不同结构的lewsi~y抗元,以ABC免疫组织化学方法观察其在人类恶性及非恶性肿瘤及正常大肠组织中的表现。这四种抗元都表现为大肠癌发生抗元。AH-6,识别结合于短琏Lewis~y抗元,大量出现于大肠癌及腺癌型息肉,但也出现于正常大肠组织。而KH-1,CC-1,CC-2三种结合于长琏Lewis~y抗元的单克隆抗体则能较好的区别癌与正常组织。仅KH-1的特异性较低(63％),而且不出现于低分化性癌。这三种抗体也结合于腺瘤型息肉,其出现率低于癌,而明显高于化生型息肉。且其出现率与腺瘤的三种瘤前病变一肿瘤大小、组织学类型及不典型增生程度分别有平行关系。而化生型息肉的改变类似于正常组织。我们认为识别结合于长琏Lewis~y的单克隆抗体可能对发现大肠癌有帮助,其在在腺瘤型息肉中的表现可能有助于判断其恶性变的倾向。
The Lewis~y anti-element is a tetra-containing sugar structure bound to type II blood type oligosaccharide glycosides-Fucl→2Gall→4(Fucl→3)GlcNAC. In this experiment, four monoclonal antibodies were used to identify the lewsi~y anti-elements with different structures, and the ABC immunohistochemical method was used to observe their performance in human malignant and non-malignant tumors and normal large intestine tissues. These four kinds of anti-elements are manifested as anti-metastatic colorectal cancer. AH-6, recognizing that it binds to short-living Lewis-y antigens, occurs in large numbers in colorectal cancer and adenocarcinoma polyps, but also in normal colon tissues. However, KH-1, CC-1, and CC-2 monoclonal antibodies that bind to Lewis-y-antigen were able to distinguish between carcinoma and normal tissues. Only KH-1 has a lower specificity (63%) and does not appear in poorly differentiated cancer. These three antibodies also bind to adenomatous polyps, which occur less frequently than cancer and are significantly higher than metaplastic polyps. And its occurrence rate and adenoma of three kinds of precancerous lesions a tumor size, histological type and atypical hyperplasia have a parallel relationship. The change in metaplastic polyps is similar to normal tissue. We believe that the recognition of the monoclonal antibody that binds to the long-term Lewis-y may be helpful for the detection of colorectal cancer, and its performance in adenoma-type polyps may help determine its tendency to malignancy.