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目的筛选糖尿病心肌病患者血清差异表达的micro RNA(mi RNA),明确mi R-186-5p和mi R-516a-5p在DCM发生发展中的作用和机制。方法选择30例DCM患者为实验组,60例年龄相当的身体健康者为正常对照组,分别从两组随机选取3例作为研究对象行micro RNA芯片初筛差异表达mi RNA,然后荧光定量q PCR验证这些mi RNA的差异表达;在高糖诱导人AC16心肌细胞损伤模型中,mi R-186-5p和mi R-516-5p的mimics和inhibitors分别干预,观察对高糖引起的心肌细胞毒性的影响。结果 micro RNA芯片检测发现,糖尿病心肌病患者血清有51个差异表达的mi RNA,经荧光定量q PCR验证为mi R-186-5p、mi R-516a-5p等8个差异表达mi RNA;显著高表达的mi R-516-5p和明显低表达的mi R-186-5p介导了高糖所致心肌细胞毒性损伤。结论糖尿病心肌病患者血清mi RNA表达谱发生改变,有8个差异表达mi RNA,mi R-516a-5p和mi R-186-5p可能参与糖尿病心肌病的心肌损伤发病过程。
Objective To screen differentially expressed micro RNA (miRNA) in serum of patients with diabetic cardiomyopathy and to clarify the roles and mechanisms of mi R-186-5p and mi R-516a-5p in the development and progression of DCM. Methods Thirty patients with DCM were selected as the experimental group and 60 healthy individuals with the same age as the normal control group. Three randomly selected miRNAs from two groups were used to screen differentially expressed mi RNA, then qPCR To verify the differential expression of these miRNAs, mimics and inhibitors of mi R-186-5p and mi R-516-5p were respectively intervened in high glucose-induced human AC16 cardiomyocyte injury model to observe the effect on myocardial cytotoxicity caused by high glucose influences. Results miRNA microarray assay showed that 51 differentially expressed mi RNAs were detected in serum of patients with diabetic cardiomyopathy and miRNAs were differentially expressed in 8 miRNAs such as mi R-186-5p and mi R-516a-5p by qRT-PCR. Highly expressed mi R-516-5p and significantly low expression of mi R-186-5p mediate cardiomyocyte-induced cytotoxic damage by high glucose. Conclusion There are 8 differentially expressed mi RNAs in patients with diabetic cardiomyopathy, and mi R-516a-5p and mi R-186-5p may be involved in the pathogenesis of myocardial injury in diabetic cardiomyopathy.