论文部分内容阅读
温室白粉虱Trialeurodes vaporariorum是为害我国蔬菜作物的重大害虫之一。本研究采用随机扩增多态性DNA(Random Amplified Polymorphic DNA,RAPD)技术对温室白粉虱进行研究,筛选出一条620bp的特异性片段(GenBank登录号为JQ690764),据此片段的测序结果设计一对特异性的序列特异性扩增区(Sequence characterized Amplified Region,SCAR)标记(Tv-F和Tv-R),可成功从室内饲养的和从不同地区田间采集的温室白粉虱的基因组DNA中扩增出一条412bp的特异性条带,而不能从供试的其他粉虱类害虫中扩增出该相应条带。单头温室白粉虱成虫的基因组DNA稀释1000倍时,该SCAR标记仍可成功扩增出预期条带,显示出极高的灵敏度。该SCAR标记对温室白粉虱的基因组DNA扩增重复性和稳定性好,且操作简便,灵敏度高,可用于样品的大规模检测,为田间温室白粉虱的快速识别鉴定及其有效防治提供了技术基础。
Greenhouse whitefly Trialeurodes vaporariorum is one of the major pests that damage our vegetable crops. In this study, Random Amplified Polymorphic DNA (RAPD) was used to study the whiteflies in the greenhouse, and a 620bp specific fragment (GenBank accession number: JQ690764) was screened. According to the sequencing results of this fragment, Specific sequence-specific Amplified Region (SCAR) markers (Tv-F and Tv-R) were successfully amplified from genomic DNA of indoor greenfly whiteflies collected in the field and collected from different areas A 412bp specific band was added, but the corresponding band could not be amplified from other whitefly pests tested. The SCAR marker can still successfully amplify the expected band when the genomic DNA of adult green leafhopper adults is diluted 1000-fold, showing an extremely high sensitivity. The SCAR marker has good repeatability and stability to genomic DNA of whitefly in greenhouse and has the advantages of simple operation and high sensitivity, which can be used for large-scale detection of samples and provides technology for rapid identification and effective control of whitefly basis.