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应用基因重组人干扰素α(IFN-α)体外诱导三株人胃癌细胞系SGC_(7901)、MGC_(803)和MKN_(45),用ABC-CELISA法检测诱导组与对照组细胞表面免疫抑制酸性蛋白Ⅱ型(IAP-2)表达量。制备人脾来源LAK细胞,MTT法检测各组LAK细胞杀伤率。结果表明,在无IFN-α存在的对照组,三株细胞均表达IAP-2,其水平较低,对LAK细胞杀伤敏感。在IFN-α诱导组,当IFN-α<1000U/ml时,三株细胞IAP-2表达量显著高于对照组(P<0.001),而LAK细胞杀伤率显著低于对照组(P<0.005);当IFN-α>1000U/ml时,这些细胞IAP-2表达量显著低于对照组(P<0.005),LAK细胞杀伤率显著高于对照组(P<0.001)。这些结果提示:IFN-α对人胃癌细胞IAP-2表达的调节可能影响癌细胞对LAK细胞杀伤敏感性。
Three human gastric cancer cell lines SGC_(7901), MGC_(803), and MKN_(45) were induced by recombinant human interferon-alpha (IFN-α) in vitro. ABC-CELISA was used to detect the cell surface immunosuppression in the induced and control groups. The expression level of acid protein type II (IAP-2). The human spleen-derived LAK cells were prepared, and the killing rate of LAK cells in each group was detected by MTT assay. The results showed that, in the control group without IFN-α, all three cells expressed IAP-2, which was low level and sensitive to LAK cell killing. In the IFN-α induction group, the expression of IAP-2 in three cells was significantly higher than that in the control group when IFN-α was less than 1000 U/ml (P<0.001), while the LAK cell killing rate was significantly lower than that in the control group (P<0.005. ); When IFN-α> 1000U/ml, the expression of IAP-2 in these cells was significantly lower than the control group (P <0.005), LAK cell killing rate was significantly higher than the control group (P <0.001). These results suggest that the regulation of IAP-2 expression in human gastric cancer cells by IFN-α may affect the sensitivity of cancer cells to LAK cell killing.