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目的对家种不同栽培条件下白花丹药材根、茎、叶中的白花丹醌进行含量测定,为建立白花丹野生变家种的栽培技术规范提供实验数据。方法采用高效液相色谱法测定家种药材根、茎、叶中白花丹醌的含量。结果在熟土和黏土中生长的药材中白花丹醌的含量高于沙土、生土;施加K肥和N肥的药材中白花丹醌的含量高于施加P肥的药材,在全光照条件下生长的药材白花丹醌含量最高;不同繁殖方式中,根、茎和叶中白花丹醌的含量变化不大。结论课题组前期研究成果表明,白花丹醌为白花丹药材抗癌和抗炎等生物活性的主要有效成分,因此,从白花丹醌含量的角度考虑,白花丹药材的最佳栽培条件为:土壤为黏土,采用扦插苗,生长过程中施加一定的N肥和K肥,光照条件为全光照,在此条件下可获得优质高产的白花丹药材。
Objective To determine the content of plumbagin in root, stem and leaf of Chinese medicinal materials Huayuandan under different cultivation conditions, and to provide experimental data for establishing the technical specifications for the cultivation of wild Huayandan. Methods HPLC method was used to determine the content of plumbagin in roots, stems and leaves of medicinal herbs. Results The content of plumbagin in medicinal plants grown in clayey soil and clayey soil was higher than that in sandy soil and earthen soil. The content of plumbagoid in the medicinal materials applying K fertilizer and N fertilizer was higher than that in P - The content of plumbagin was the highest in the growing medicinal materials. The content of plumbagin in roots, stems and leaves did not change much in different breeding methods. Conclusions The previous research results of the research group showed that plumbagin is the main active component of anti-cancer and anti-inflammatory biological activity of the Chinese white Huadan, so the best cultivation conditions of the Chinese medicinal materials are soil For clay, cutting seedlings, the growth process to impose a certain amount of N fertilizer and K fertilizer, light conditions for the whole light, in this condition can be obtained high-quality and high-yield white Huadu herbs.