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目的:探讨ki67、c-erbB-2在结肠癌及腺瘤中的表达及意义。方法:采用免疫组化S-P法检测58例结肠癌,36例结肠腺瘤,20例正常结肠组织,分别进行了ki67蛋白、c-erbB-2蛋白的检测。结果:ki67在腺癌中的阳性率及表达强度最高,显著高于腺瘤(P<0.01),腺瘤的中、重度不典型增生明显高于轻度不典型增生(P<0.01)。c-erbB-2在腺癌中的阳性表达强度和表达率与正常黏膜及腺瘤相比差异有显著性(P<0.01),有淋巴结转移者明显高于无淋巴结转移者(P<0.01)。结论:ki67的阳性表达及表达强度对诊断腺癌和监视腺瘤癌变有重要意义。c-erbB-2的阳性表达与结肠癌的发生、发展密切相关,可作为判断结肠癌恶性程度、淋巴结转移及预后的重要参考指标。
Objective: To investigate the expression and significance of ki67 and c-erbB-2 in colon cancer and adenoma. Methods: The expressions of ki67 protein and c-erbB-2 protein in 58 cases of colon cancer, 36 cases of colon adenoma and 20 cases of normal colon tissues were detected by immunohistochemical S-P method. Results: The positive rate and the expression intensity of ki67 in adenocarcinoma were significantly higher than those in adenoma (P <0.01). The moderate and severe dysplasia of adenoma was significantly higher than that of mild dysplasia (P <0.01). The positive expression intensity and the expression rate of c-erbB-2 in adenocarcinoma were significantly different from those in normal mucosa and adenoma (P <0.01), and those with lymph node metastasis were significantly higher than those without lymph node metastasis (P <0.01) . Conclusion: The positive expression of ki67 and the intensity of expression are important for the diagnosis of adenocarcinoma and the surveillance of adenoma. The positive expression of c-erbB-2 is closely related to the occurrence and development of colon cancer, which can be used as an important reference index to judge the degree of malignancy, lymph node metastasis and prognosis of colon cancer.