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建立了LC-MS/MS法测定贝母素甲在家兔血浆中的含量,研究灌胃及静注给予贝母素甲后家兔体内的药动学特征。以卡马西平为内标,采用C_(18)色谱柱,以乙腈-10 mmol/L甲酸铵溶液(35:65)为流动相,ESI源正离子模式,多反应离子(MRM)检测,监测离子对m/z 432.4→414.4(贝母素甲)和m/z 237.1→194.2(卡马西平)。贝母素甲在0.8～200 ng/ml浓度范围内线性关系良好,提取回收率88.5%～98.3%,日内、日间RSD均小于15%。家兔灌胃及静注给予贝母素甲后体内分别呈二室及三室开放模型,主要药动学参数如下:c_(max)(48.31±7.40)和(84.39±15.39)ng/ml,AUC_(0→∞)(270.08±80.17)和(50.72±14.02)ng·ml~(-1)·h~(-1),t_(1/2)(6.38±3.11)和(2.19±1.07)h。
The LC-MS / MS method was used to determine the content of Fritillaria in rabbits plasma. The pharmacokinetic characteristics of Fritillaria in rabbits after intragastric and intravenous injection of Fritillaria were studied. Carbamazepine was used as an internal standard, C 18 column, acetonitrile-10 mmol / L ammonium formate solution (35:65) was used as the mobile phase, ESI source positive ion mode and multi-reactive ion (MRM) Ion pair m / z 432.4 → 414.4 (pealin) and m / z 237.1 → 194.2 (carbamazepine). The linear range was 0.8 ~ 200 ng / ml. The average recoveries were 88.5% -98.3%. The intra-day and inter-day RSD were all less than 15%. The main pharmacokinetic parameters were as follows: c max (48.31 ± 7.40) and (84.39 ± 15.39) ng / ml, AUC_ (0 → ∞) (270.08 ± 80.17) and (50.72 ± 14.02) ng · ml -1 · h -1, t 1/2 (6.38 ± 3.11) and (2.19 ± 1.07) h respectively .