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以大豆下胚轴为试验材料,研究了绿原酸(CGA)及其与植物生长调节剂(IAA、IBA)共同作用对大豆下胚轴不定根发生的影响。结果表明,当CGA施用量达到200μmol/L及400μmol/L时,大豆下胚轴生根率分别显著降低了17.8%和26.7%,平均根长分别显著降低了18.7%和35.4%;当植物生长调节剂施用量为2μmol/L时,可显著缓解CGA(100μmol/L)对大豆不定根的抑制作用,使其生根率分别提高12.5%和20.0%,随着生长素施用量的进一步增加(10~50μmol/L),其生根率显著下降;大豆不定根培养24 h内,对照下胚轴基部木质素相对含量降低,CGA处理大豆的木质素相对含量升高。高浓度CGA对大豆不定根形成具有抑制作用,其通过与木质素、生长素的共同作用,实现对大豆不定根发生的调控。
The effects of chlorogenic acid (CGA) and its combination with plant growth regulator (IAA, IBA) on the adventitious root formation of soybean hypocotyls were studied using the hypocotyls of soybean as experimental material. The results showed that the rooting rate of hypocotyls of soybean decreased significantly by 17.8% and 26.7% when CGA application reached 200μmol / L and 400μmol / L, respectively, and the average root length decreased by 18.7% and 35.4% respectively. When plant growth regulators At the dosage of 2 μmol / L, the inhibitory effect of CGA (100 μmol / L) on adventitious rooting of soybean was significantly alleviated, and its rooting rate was increased by 12.5% and 20.0%, respectively. With the further increase of auxin dosage / L), the rooting rate decreased significantly. Within 24 h of adventitious root culture, the relative content of lignin at the base of hypocotyls decreased and the relative content of lignin in CGA-treated soybean increased. High concentrations of CGA can inhibit the formation of adventitious roots in soybean, and through the interaction with lignin and auxin, the regulation of adventitious root formation in soybean can be achieved.