论文部分内容阅读
目的探讨牛磺酸对大鼠肝脏缺血再灌流损伤的作用及机制。方法48只实验大鼠随机分为三组假手术组(A组)、缺血-再灌流组(B组)和给予牛磺酸后缺血-再灌流组(C组)。检测再灌流30min的肝组织钙含量、丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-PX)活力。并同步观察肝组织光镜和电镜下的形态变化。结果B组肝组织钙含量和MDA含量明显高于A组(P<0.01),而GSH-PX活力低于A组(P<0.01)。C组肝组织钙和MDA含量低于B组(P<0.01,P<0.05),而GSH-PX活力高于B组(P<0.05)。肝损伤病理改变C组轻于B组(P<0.05)。结论牛磺酸能显著减轻大鼠肝脏缺血再灌流损伤,其机制与减轻钙超载、抑制氧自由基生成和增强氧自由基清除有关。
Objective To investigate the effect and mechanism of taurine on hepatic ischemia-reperfusion injury in rats. Methods Forty eight experimental rats were randomly divided into three groups: sham operation group (A group), ischemia - reperfusion group (B group) and taurine - ischemia - reperfusion group (C group). The content of calcium, malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) activity in liver tissue were detected 30 min after reperfusion. The morphological changes of liver tissue under light and electron microscopes were observed synchronously. Results The content of calcium and MDA in liver tissue of group B were significantly higher than that of group A (P <0.01), while the activity of GSH-PX was lower than that of group A (P <0.01). The contents of calcium and MDA in liver tissue in group C were lower than those in group B (P <0.01, P <0.05), while the activities of GSH-PX in liver were higher than those in group B (P <0.05). The pathological changes of liver injury in group C were lighter than those in group B (P <0.05). Conclusion Taurine can significantly reduce the hepatic ischemia-reperfusion injury in rats. The mechanism is related to the reduction of calcium overload, inhibition of oxygen free radical production and enhancement of oxygen free radical scavenging.