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目的采用基于LARS算法的非线性多元回归方法预测最优变量组合,验证该组合的有效性,并进一步探讨芪苈强心胶囊对实验性心衰大鼠心功能保护的作用机制。方法 72只Sprague Dawley(SD)大鼠随机分为6组,假手术组,模型对照组,阳性对照组,芪苈强心胶囊组合物(QLQX)低、中、高剂量组,连续7次给药后,结扎冠状动脉复制实验性心衰模型,4 h后经右颈总动脉行左心室插管,测定左心室压峰值(LVP)等心功能指标;取血清测定超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH);取心肌组织Western Blot检测过氧化物酶体增殖物激活受体(PPAR)γ的表达。结果与模型对照组比较,QLQX可提高左心室压力(LVP)(P<0.05)、左心室内压最大上升速率(+LVdp/dtmax)(P<0.05),降低左心室舒张末期压(LVEDP)(P<0.05);还可提高SOD、GSH活性,降低MDA含量,QLQX高剂量降低幅度达20%(P<0.01);Western Blot检测出QLQX的PPARγ表达增高,以高剂量作用显著。结论采用LARS算法得到的芪苈强心胶囊最佳配伍对实验性心衰大鼠心功能有改善作用,其作用与清除自由基、提高心肌细胞抗氧化能力有关。
Objective To predict the optimal combination of variables by nonlinear multiple regression based on LARS algorithm to verify the validity of the combination and to further explore the mechanism of Qili Qiangxin capsule on the protection of cardiac function in experimental heart failure rats. Methods Seventy-two Sprague Dawley rats were randomly divided into 6 groups: sham operation group, model control group, positive control group, Qi Qiang Qiang Xin Capsule Composition (QLQX) low, medium and high dose group After the medicine was given, the model of experimental heart failure was reproduced by ligating the coronary artery. After 4 hours, the left ventricular pressure was measured by left ventricular cannulation through the right common carotid artery. The serum levels of superoxide dismutase (SOD) ), Malondialdehyde (MDA) and glutathione (GSH) were measured. The expression of peroxisome proliferator activated receptor (PPAR) γ was detected by Western Blot. Results Compared with the model control group, QLQX increased left ventricular pressure (LVP) and left ventricular pressure (LVdp / dtmax) (P <0.05) and decreased left ventricular end diastolic pressure (LVEDP) (P <0.05). It also increased the activity of SOD and GSH and decreased the content of MDA. The QLQX high dose decreased by 20% (P <0.01). The expression of PPARγ in QLQX was higher than that in QLQX. Conclusion The best compatibility of Qili Qiangxin Capsule obtained by LARS algorithm can improve cardiac function in experimental heart failure rats, and its effect is related to eliminating free radicals and improving anti-oxidative ability of cardiomyocytes.