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肿瘤细胞表型差异是临床药物敏感性、抗原性、转移性、潜伏性和进展速度等差异的主要原因。为了弄清恶性表型差别的表现、相关性及调节机理、我们从胃癌MGC 803系分离了单细胞亚系,各亚系按克隆过程的时间差别归纳分组,选快组M 17、中组M 6和慢组M 3进行比较。软琼脂生长实验表明三者的恶性程度有显著差别;M17恶性度最高,M3最低,M6居中。与其它表型联系比较,证明细胞生长速度、细胞微丝、微管骨架破坏及细胞通讯功能抑制都与锚着不依赖性生长的恶性特征有平行相关性。同时,M 17和M 3原癌基因表达有明显差别。
The phenotypic difference of tumor cells is the main reason for the differences in clinical drug sensitivity, antigenicity, metastasis, latency, and progression velocity. In order to clarify the manifestations, correlations, and regulatory mechanisms of malignant phenotypic differences, we isolated single cell sublines from the gastric cancer MGC 803 line. Each subline was grouped according to the time difference of the cloning process, and the fast group M 17 and middle group M were selected. 6 and slow group M 3 are compared. Soft agar growth experiments showed that there was a significant difference in the degree of malignancy between the three groups; M17 had the highest malignancy, M3 had the lowest, and M6 had the middle. Compared with other phenotypes, it was demonstrated that cell growth rate, cell microfilament, microtubule cytoskeleton destruction, and inhibition of cell communication function all have parallel correlation with anchorage-independent growth malignant characteristics. At the same time, the expression of M 17 and M 3 proto-oncogenes was significantly different.