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本文报道了普通野生稻稻褐飞虱抗源的杂交利用技术及其获得的一大批抗性创新种质和育种品系。研究了杂交后代性状分离、不同杂交方式、抗性鉴定时期和花药培养的抗性育种效果,证明了复交和回交方式、花药培养获得纯合体、以及早期(F2)抗性鉴定是在育种上利用的关键技术措施。本项目还配制了大量(293个)杂交组合,进行稻褐飞虱抗性创新品系的选育工作,对选育出的493个遗传稳定品系进行抗性鉴定、运用RAPD标记对筛选出的143份抗性育种品系进行分子标记多态性分析,从中首次获得了一大批(120份)具有DNA分子标记多态性(遗传多样性)的抗性创新种质,为今后培育抗性品种打下基础。研究还初步培育出5个具有生产应用价值的高产(或优质)抗稻褐飞虱育种品系和杂交稻组合。
In this paper, we report the hybrid utilization of common wild rice (Oryza sativa L.) resistance to brown planthopper (Oryza sativa L.) and its accessions to a large number of innovative germplasm and breeding lines. The effects of segregation, hybridization, resistance identification and anther culture on hybrid progenies were studied. The results showed that the methods of multiple cross and backcross, the homozygote of anther culture, and the identification of early resistance (F2) The use of key technical measures. In this project, a large number of (293) hybrid combinations were prepared for the breeding of the resistant lines of the brown planthopper, and the resistance of 493 stable genetic lines were identified. RAPD markers were used to screen the 143 resistant lines Sexual breeding lines were analyzed by molecular marker polymorphism. For the first time, a large number of (120) innovative resistant germplasms with DNA molecular marker polymorphism (genetic diversity) were obtained, laying the foundation for breeding resistant varieties in the future. The study also initially bred five high yield (or good quality) resistance to BPH breeding lines and hybrid rice combinations with production value.