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目的、方法：用高效液相色谱分析、比较豚鼠咽鼓管和肺表面活性物质中磷脂的组成成分和含量，为人工合成咽鼓管表面活性物质，预防和治疗中耳炎提供理论依据。结果：咽鼓管和肺表面活性物质中均检测出了磷脂酰胆碱（ＰＣ）、磷脂酰肌醇（ＰＩ）、磷脂酰丝氨酸（ＰＳ）、磷脂酸（ＰＡ）、磷脂酰乙醇胺（ＰＥ）、神经鞘磷脂（ＳＰ），每４只咽鼓管中各磷脂含量（ｇ）及所占百分比：ＰＥ：１２３．４６４０．６６，（４２８）％；ＳＰ：６７．９５１７．９４，（２１４）％；ＰＳ：４８．５７土１８．８４，（１６４％）；ＰＣ：３２．６０１３．１７，（１１４）％；ＰＩ：２２．９４５．７３，（８士２）％；ＰＡ：２．９０士０．００，（１０．３）％．而１个肺中：ＰＣ：５６３．８２士７４．５，（６８土４）％；ＰＥ：９５．９２士２０．３０，（１１士２）％；Ｐｌ：６８．３３士１４．６３，（８士１）％；ＰＳ：５１．４３士１４．２６，（６２）％；ＳＰ：３４．０４土６．１３，（４士１）％：ＰＡ：１６．８３ｌ．６１，（２土０）％。结论：咽鼓管表面活性物质中磷腊成分含量不同于肺，提示体内不同部位的表面活性物质可能各有特殊的功能作用。
OBJECTIVE, METHOD: To compare the composition and content of phospholipids in the eustachian tube and pulmonary surfactant of guinea pig with high performance liquid chromatography (HPLC), to provide a theoretical basis for the synthesis of eustachian tube surface active substances and prevention and treatment of otitis media. Results: The levels of phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidic acid (PA) and phosphatidylethanolamine (PE) were all detected in eustachian tube and pulmonary surfactant , Sphingomyelin (SP), and the content of each phospholipid in four eustachian tubes (g): PE: 123.4640.66, (428)%; SP: 67.9517.94, (214) %; PS: 48.57 ± 18.84, (164%); PC: 32.6013.17, (114)%; PI: 22.945.73, (8 ± 2)%; 0.00% (10.3%). While in one lung: PC: 563.82 ± 74.5, (68 ± 4%); PE: 95.92 ± 20.30, (± 1 ± 2)%; Pl: 68.33 ± 14.63, (8 ± 1)%; PS: 51.43 ± 14.26, (62)%; SP: 34.04 ± 6.13, (4 ± 1)%: PA: 16.83l. 61, (2 soil 0)%. CONCLUSION: The content of phosphorus wax in the eustachian tube surface active substance is different from that in the lung, suggesting that the surface active substances in different parts of the body may each have special function.