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目的本研究通过对BP1mRNA在肺癌组织中的表达状况的研究,探讨BP1mRNA在肺癌中的表达水平以及和肺癌的临床关系。方法采用逆转录聚合酶链反应(RT-PCR)技术,检测46例肺癌病人的癌组织,相应的癌旁组织和正常组织标本中BP1mRNA的表达程度及意义。结果在46组标本中,肺癌组织中有36例BP1mRNA呈阳性表达(78.26%),癌旁组织6例呈阳性表达(13.04%),正常组织中未检出阳性表达。癌组织,癌旁组织和正常组织中BP1mRNA的表达率之间存在显著性差异(P<0.01)。癌旁组织和正常组织中BP1基因的表达率之间无显著性差异(P>0.05)。在21例高分化标本中13例呈中高表达,25例低分化标本中22例呈中高表达,两者之间存在显著性差异(P<0.01);在20例Ⅰ-Ⅱ期肺癌标本中12例BP1基因呈中高表达,26例Ⅲ-Ⅳ期标本中15例呈中高表达,两者之间无显著性差异(P>0.05)。BP1基因的表达程度在24例鳞癌中21例呈高表达,在22例腺癌中19例呈高表达,两者之间无显著性差异(P>0.05)。结论BP1mRNA在肺癌组织中的表达显著上调;BP1基因的表达可能与肺癌分化程度有关,与病理类型和TNM分期无关。
OBJECTIVE: To investigate the expression of BP1 mRNA in lung cancer and the clinical relationship with lung cancer by studying the expression of BP1 mRNA in lung cancer. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression and significance of BP1 mRNA in 46 cases of lung cancer tissues, corresponding paracancerous tissues and normal tissues. Results Among the 46 specimens, 36 specimens of lung cancer showed positive expression of BP1 mRNA (78.26%), 6 specimens of paracancerous tissues (13.04%) and no positive expression in normal tissues. There was a significant difference (P <0.01) between the expression of BP1mRNA in cancer tissues, adjacent tissues and normal tissues. There was no significant difference in the expression rate of BP1 gene between adjacent tissues and normal tissues (P> 0.05). Among the 21 cases of well differentiated specimens, 13 cases were highly expressed, 22 of 25 poorly differentiated specimens were overexpressed, with significant difference (P <0.01). Among 20 cases of stage Ⅰ-Ⅱ lung cancer, 12 The BP1 gene was highly expressed in 15 cases of mid-high expression in 26 cases of stage Ⅲ-Ⅳ, which showed no significant difference (P> 0.05). The expression of BP1 gene was highly expressed in 21 cases of squamous cell carcinoma of 24 cases and 19 cases of 22 cases of adenocarcinoma, with no significant difference (P> 0.05). Conclusion The expression of BP1 mRNA in lung cancer tissues is significantly up-regulated. The expression of BP1 gene may be related to the degree of lung cancer differentiation, but not to the pathological type and TNM stage.