论文部分内容阅读
目的探讨前列腺癌中XAF1 mRNA表达及DNA甲基化抑制剂对其表达的影响。方法培养前列腺癌细胞株LNCaP、DU145和PC3及肾癌细胞株A498,提取正常人外周血单核细胞(PBMC)、A498和PBMC用作阳性对照。搜集临床10例前列腺癌及邻近前列腺组织作对照。RT-PCR法检测各细胞株和正常人外周血单核细胞中XAF1 mRNA表达,甲基化特异性PCR法检测甲基化酶抑制剂(5’-aza)处理前后前列腺癌细胞株LNCaP、DU145中XAF1 mRNA表达。结果 LNCaP和DU145中XAF1 mRNA的表达水平低于肾癌细胞株A498,PC3中XAF1mRNA不表达。8例患者中有6例XAF1 mRNA在癌组织中的表达低于邻近前列腺组织。使用5’-aza处理后的LNCaP和DU145中XAF1 mRNA均以全长形式表达。结论 XAF1 mRNA在LNCaP和DU14中均为低表达,在PC3不表达。DNA 5’-aza可诱导前列腺癌细胞株中XAF1 mRNA的全长表达。
Objective To investigate the expression of XAF1 mRNA in prostate cancer and the effect of DNA methylation inhibitor on it. Methods Prostate cancer cell lines LNCaP, DU145 and PC3 and renal cell carcinoma cell line A498 were cultured and PBMCs were extracted. A498 and PBMC were used as positive control. Collect clinical 10 cases of prostate cancer and adjacent prostate tissue as a control. The expression of XAF1 mRNA was detected by RT-PCR in peripheral blood mononuclear cells (PBMCs) and normal human PBMCs. The methylation-specific PCR was used to detect the expression of XAF1 mRNA in prostate cancer cell lines LNCaP and DU145 In XAF1 mRNA expression. Results The expression level of XAF1 mRNA in LNCaP and DU145 was lower than that in renal cell carcinoma cell line A498, while the expression of XAF1 mRNA in PC3 was not expressed. XAF1 mRNA expression in 6 of 8 patients was lower than that in adjacent prostate tissue. Both LNCaP treated with 5’-aza and XAF1 mRNA in DU145 were expressed in full-length form. Conclusion XAF1 mRNA is low expressed in LNCaP and DU14, but not expressed in PC3. DNA 5’-aza induces the full-length expression of XAF1 mRNA in prostate cancer cell lines.