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从山西不同生态条件下，采集豆科植物根瘤，经分离、纯化、回接原寄主，获得来自３１属７１种豆科植物寄主的纯根瘤菌株２１２株．对１５４株进行了ＢＴＢ反应和生长速度测定，在ＹＭＡ培养基上产酸快生型占９７．４％．选用了其中５０株，以快生型苜蓿根瘤菌（Ｒ．ｍｅｌｉｌｏｔｉ）和豌豆根瘤菌（Ｒ．ｌｅｇｕｍｉｎｏｓａｒｕｍ）各３株为ＣＫ，进行了其它生理生化鉴定．结果表明，５６株参试菌株均不产生３－酮基乳糖．山西分离的菌株能在蛋白胨肉汤上生长的１３株；能利用柠檬酸盐的７株；石蕊牛奶反应，产酸的１２株，其中酸凝的８株，产碱的３０株，其中胨化的１１株，石蕊还原的７株．唯一碳源和氮源利用比ＣＫ更广泛．对温度的适应范围宽．比ＣＫ有更强的耐盐、耐碱和对抗菌素的耐受能力
Under different ecological conditions in Shanxi, the nodules of leguminous plants were collected, separated, purified and returned to the original host plants to obtain 212 pure rhizobial strains from 71 legumes of 31 genera. 154 strains were subjected to BTB reaction and growth rate determination, and 97.4% of them were acidogenic in YMA medium. Fifty of them were selected, and other physiological and biochemical characteristics were identified by using three fast-growing alfalfa (R. meliloti) and three strains of R. leguminosarum as CK. The results showed that none of 56 tested strains produced 3-keto-lactose. Strain isolated in Shanxi can grow on the peptone broth 13; can use citrate 7; litmus milk reaction, acid-producing 12, of which 8 acid-base, 30 of alkali-producing peptone The 11 strains, litmus reduction of 7 strains. The only sources of carbon and nitrogen are more widely used than CK. Wide range of temperature adaptation. More resistant to salt than salt CK, alkali and antibiotic tolerance