论文部分内容阅读
目的 了解母婴传播后母子体内 HBV复制程度差异与 C区启动子变异的关系。方法 对 8对复制程度不同的HBV C区启动子基因进行聚合酶链反应（PCR）,应用TA克隆技术构建重组质粒pGEM—CP,每例患者选2个双酶切鉴定正确的克隆测序并分析。结果 HBV复制状态,子高母低组平均变异数于为5.33±1.53,母为9.33±3.06;子低母高组于为8.25±4.27,母为5.00±1.41。母子低复制者C区启动子变异数及变异位点数明显高于高复制者,变异主要集中在BCP和Kunitz类丝氨酸蛋白序列区域,而同复制状态下母子间差异不大。结论母婴传播后母子体内HBV低复制状态与C区启动子变异有关,可能与机体的生长发育状态无关。
Objective To understand the relationship between HBV replication and mother-to-child C mutation in mothers and infants after mother-to-child transmission. Methods Eight pairs of HBV C region promoter genes with different degrees of replication were amplified by polymerase chain reaction (PCR). The recombinant plasmid pGEM-CP was constructed by TA cloning technique. Two double enzyme digestion was performed in each patient to identify the correct cloning and sequencing . Results In HBV replication status, the average number of variation in subglottic maternal low group was 5.33 ± 1.53, the mother was 9.33 ± 3.06; the sub-low maternal high group was 8.25 ± 4.27 and the mother was 5.00 ± 1.41. The number of motifs and the number of variation loci in the C region of low-copy gene mothers were significantly higher than those of high-copy ones, and the variation was mainly concentrated in serine protein sequence regions of BCP and Kunitz. Conclusion The mother-to-child HBV replication status is related to the mutation of promoter region C in mother-to-child transmission, which may not be related to the growth and development of the body.