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目的:研究肉苁蓉总苷(GCs)对阿尔茨海默病(AD)模型大鼠的保护作用。方法:大鼠采用脑立体定向技术向海马背侧处注射凝聚态β-淀粉样蛋白(Aβ25-35)以复制大鼠AD模型。实验分为假手术(等容生理盐水)、模型(等容生理盐水)、维生素E(40mg/kg)与GCs高、中、低剂量(160、80、40mg/kg)组。于复制模型前4d开始灌胃给药,每天1次,连续14d。以被动回避性跳台实验和电迷宫实验法检测大鼠学习记忆能力;制备大鼠海马区组织匀浆及脑组织切片,检测大鼠海马区组织乙酰胆碱酯酶(AchE)活性,电子显微镜观察大鼠海马CA1区细胞内Ca2+含量。结果:与假手术组比较,模型组大鼠反应时间显著延长、错误次数显著增多(P<0.01),海马组织AchE活性显著增强(P<0.01),海马CA1区中细胞内Ca2+含量明显增加。与模型组比较,GCs高、中、低剂量组大鼠反应时间显著缩短、错误次数显著减少(P<0.01),海马组织中AchE活性显著减弱(P<0.01或P<0.05),海马CA1区中细胞内Ca2+含量明显降低。结论:GCs能够降低AD模型大鼠海马组织中AchE活性和Ca2+含量,维持脑内乙酰胆碱(Ach)的正常水平并提高学习记忆能力。
Objective: To study the protective effects of glycosides of Cistanche (GCs) on Alzheimer’s disease (AD) model rats. Methods: The rat model of AD was replicated by injecting condensed β-amyloid (Aβ25-35) into the dorsal part of hippocampus by stereotactic technique. The experiment was divided into sham operation (isosceles normal saline), model (isosceles normal saline), vitamin E (40mg / kg) and high, medium and low dose of GCs (160, 80, 40mg / kg) 4d before the replication model began gavage, 1 day, for 14 days. The learning and memory abilities of rats were detected by passive avoidance step-down test and electric maze test. Homogenate and brain tissue sections of hippocampus were prepared and the activity of acetylcholinesterase (AchE) in hippocampus of rats was measured. Electron microscopy Intracellular Ca2 + content in hippocampal CA1 area. Results: Compared with the sham operation group, the response time of model group was significantly longer, the number of errors was significantly increased (P <0.01), the activity of AchE in hippocampus was significantly increased (P <0.01), and the content of Ca2 + in hippocampal CA1 region was significantly increased. Compared with the model group, the response time of GCs in high, middle and low dose groups was significantly shortened, the number of errors significantly decreased (P <0.01), AchE activity in hippocampus significantly decreased (P <0.01 or P <0.05) The intracellular Ca2 + content was significantly reduced. CONCLUSION: GCs can reduce AchE activity and Ca2 + content in hippocampus of AD model rats, maintain the normal level of acetylcholine (Ach) in brain and improve learning and memory abilities.