论文部分内容阅读
目的 :探讨孕牛血清中早孕因子 (EPF)的生化特性。方法:对孕牛 (妊娠 4～ 5个月 )血清进行标准化处理 ,采用硫酸铵分级沉淀、DEAE- Sepharose、CM- Sepharose柱层析、玫瑰花环抑制滴度试验、SDS- PAGE和等电聚焦等技术对 EPF进行生化特性的研究。 结果 :在孕牛血清中分离出分子量分别为 2 1、6 7k D两条区带的蛋白质 ,以 2 1k D区带蛋白质活性最强 ,其等电点为 6 .80。结论 :孕牛血清中存在着分子量为 2 1、6 7k D的蛋白质 ,均具有EPF特性 ,2 1k D蛋白等电点为 6 .80。
Objective: To investigate the biochemical characteristics of early pregnancy factor (EPF) in pregnant cattle serum. Methods: The serum of pregnant cows (4 ~ 5 months of gestation) was normalized by ammonium sulfate fractionation, DEAE-Sepharose, CM-Sepharose column, rosette inhibition titer, SDS-PAGE and isoelectric focusing Technology biochemical characteristics of EPF research. Results: Two proteins with molecular weight of 2 1,6 7 kD were separated from the serum of pregnant calf. The protein in 21 kD band was the strongest and the isoelectric point was 6.80. CONCLUSION: There are proteins with a molecular weight of 2 1, 6 7k D in the serum of pregnant calves, all of which have EPF characteristics. The 21kD isoelectric point is 6.80.