论文部分内容阅读
目的探讨大鼠实验性隐睾对睾丸间质细胞数量、形态及相关功能的影响。方法采用手术诱导方法制作大鼠(6月龄)双侧隐睾模型(每组6只),分别于术后第3天、7天、20天和28天取左侧睾丸入Bouin液固定,假手术组作为对照组。应用体视学方法检测睾丸间质细胞的数量、形态变化;应用免疫组织化学检测细胞分裂周期蛋白25A(CDC25A)、干细胞因子(SCF)、雄激素受体(AR)和雌激素受体α(ERα)的表达变化。结果隐睾术后睾丸间质体积分数明显增加,但睾丸间质的绝对体积无明显变化。间质细胞数量和光镜形态也无明显变化。免疫组织化学方法显示,CDC25A和SCF在间质细胞区域强表达,隐睾对其表达无明显影响;AR在间质细胞表达较弱,位于细胞质内,各组之间表达强度无明显变化;ERα在对照组睾丸间质细胞内表达较弱,间质细胞核基本无阳性着色,但随着隐睾时间的延长,间质细胞核阳性表达比例明显升高,在隐睾术后第28天时达到93.6%。结论大鼠实验性隐睾对睾丸间质细胞的数量和光镜形态无明显影响,但对其甾体类激素合成及相关调节功能有明显影响。
Objective To investigate the effects of experimental cryptorchidism on the number, morphology and related functions of testicular stromal cells in rats. Methods Bilateral cryptorchidism model (6 in each group) was made by surgical induction method. Left testes were placed into Bouin fluid fixation on the 3rd, 7th, 20th and 28th day after operation. Sham-operated group served as control group. The quantity and morphology of testicular interstitial cells were detected by stereological methods. Immunohistochemistry was used to detect the expression of CDC25A, SCF, AR and estrogen receptor alpha ERα) expression changes. Results The percentage of testicular interstitial volume increased significantly after cryptorchidism, but there was no significant change in the absolute volume of testicular interstitium. There was no significant change in the number of stromal cells and light microscope. Immunohistochemistry showed that CDC25A and SCF were strongly expressed in interstitial cells, while cryptorchidism had no effect on the expression of CDC25A and SCF. The expression of AR in interstitial cells was weak and located in the cytoplasm. There was no significant change in the expression of ERα In control group, the expression of interstitial cells in testicular stromal cells was weak, and the interstitial nuclei were almost not stained positively. However, with the extension of cryptorchidism time, the positive expression rate of interstitial nuclei was significantly increased, reaching 93.6 days after operation on the 28th day %. Conclusion The experimental cryptorchidism has no significant effect on the number of testicular stromal cells and the morphology of light microscope, but significantly affects the steroid hormone synthesis and related regulatory functions.