论文部分内容阅读
为了比较病毒感染与高脂饮食造成家兔的血管组织病理变化。将 6 0只家兔分为对照组 (正常对照组 )、灭活病毒组、高脂饮食组、病毒感染组四组 ,随后给予相应处理。 6 0d后取动物血管组织 ,以免疫组织化学方法检测组织内病毒抗原作为病毒感染的证据 ,分析高脂饮食与病毒感染损伤血管内膜的光镜和电镜病理学特点。结果发现 ,对照组动物血管组织无论光镜或电镜均未见明显异常。高脂组动物光镜下在血管查到脂质沉积 ,红细胞粘附 ;扫描电镜可见内皮细胞破损 ,有红细胞和白细胞粘附于内皮细胞表面 ;透射电镜可见动物血管内皮细胞、平滑肌细胞和巨噬细胞内有脂滴。在病毒组动物 ,光镜检查发现血管组织损伤主要为内皮细胞的变性、坏死和脱落 ,部分血管腔内有密度较高的呈丝状、颗粒状似“血栓样堆积物” ;扫描电镜发现内皮细胞表面有丝状物粘附 ;透射电镜检查发现血管基底膜增宽 ,厚度不均 ,内皮细胞微突增多 ,核呈分叶状 ,异染色质积聚 ,靠核膜处电子密度增高 ,核膜呈皱折状。在一份血管标本的平滑肌细胞浆内查到可疑病毒颗粒 ;免疫组织化学法在多组织细胞、尤其是血管内皮细胞查到人巨细胞病毒抗原。实验结果提示 ,病毒感染与高脂饮食造成的血管内皮细胞损伤病理改变不同。
In order to compare the virus infection with high-fat diet caused by pathological changes in rabbit vascular tissue. Sixty rabbits were divided into four groups: control group (normal control group), inactivated virus group, high-fat diet group and viral infection group, followed by the corresponding treatment. Sixty days later, the vasculature of the animals was taken, and the virus antigens in the tissues were detected by immunohistochemistry as the evidence of virus infection. The pathological features of the intima of injured vessels were analyzed by light and electron microscopy. The results showed that the control group of animal vascular optical microscope or electron microscopy showed no significant abnormalities. Lipid deposition and erythrocyte adhesion were observed in blood vessels in the high-fat group under light microscope. Endothelial cells were damaged by scanning electron microscopy, and erythrocytes and leukocytes adhered to the surface of endothelial cells. Transmission electron microscope showed that vascular endothelial cells, smooth muscle cells and macrophages Intracellular lipid droplets. In the virus group animals, light microscopy found that vascular tissue damage mainly denaturation, necrosis and loss of endothelial cells, part of the vascular cavity with a higher density was filiform, granular like “thrombus-like deposits”; scanning electron microscopy found that the endothelium Transmission electron microscopy showed that the vascular basement membrane was widened and the thickness was not uniform. The number of microvessels in endothelial cells increased. The nuclei showed lobulated and heterochromatin accumulation. The electron density at the nuclear membrane increased. The nuclear membrane Was crumpled. Viruses were found in the smooth muscle cytoplasm of a blood vessel specimen. Immunocytochemistry detected human cytomegalovirus antigens in multiple tissue cells, especially in vascular endothelial cells. The experimental results suggest that the pathological changes of vascular endothelial cell injury caused by virus infection and high-fat diet are different.