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目的 血管活性肠肽 (VIP)与肿瘤关系密切 ,VIP可促进和 /或抑制一些肿瘤生长 ,而且一些肿瘤细胞有VIP的自分泌调节作用。VIP对胃癌生长的影响 ,胃癌细胞是否分泌VIP及存在VIP自分泌调节作用尚有争议。研究肠型胃腺癌及不典型增生组织中VIPmRNA的表达情况 ,试图探讨VIP在胃腺癌发生发展中的作用。方法 通过RT PCR方法 ,检测 1 5例正常胃窦黏膜、1 6例不典型增生胃黏膜及 2 0例肠型胃腺癌组织中VIPmRNA的表达量 ,通过与恒定表达的β actin(内参照 )mRNA表达量比较 ,计算出VIPmRNA与 β actinmRNA表达量的积分光密度比值 (V/B) ,以V/B值反映各组织中VIPmRNA表达量的高低。结果 肠型胃腺癌组织中V/B值明显高于不典型增生胃黏膜及正常胃窦黏膜 ,其V/B值分别为 :1 .452 2± 0 .32 82 ,0 .962 3± 0 .2 2 5 0 ,0 .951 3± 0 .2 66 9,差异有非常显著性 (P<0 .0 1 )。正常胃窦黏膜与不典型增生胃黏膜中 ,V/B值的差异无显著性 (P >0 .0 5)。结论 肠型胃腺癌组织VIPmRNA表达量明显高于不典型增生胃黏膜 ;VIP可能在肠型胃腺癌的发生发展中起重要的作用。
Objective Vasoactive intestinal peptide (VIP) is closely related to tumors. VIP can promote and/or inhibit the growth of some tumors, and some tumor cells have VIP autocrine regulation. The effect of VIP on the growth of gastric cancer, whether gastric cancer cells secrete VIP and the existence of VIP autocrine regulation are still controversial. To investigate the expression of VIP mRNA in intestinal gastric adenocarcinoma and atypical hyperplasia, and to explore the role of VIP in the development of gastric adenocarcinoma. Methods RT-PCR method was used to detect the expression of VIP mRNA in 15 cases of normal gastric antral mucosa, 16 cases of atypical hyperplasia gastric mucosa and 20 cases of intestinal type gastric adenocarcinoma. The expression of β-actin (internal reference) mRNA was determined by RT PCR. Comparing the expression amounts, the integrated optical density ratio (V/B) of the VIP mRNA and β actin mRNA expression was calculated, and the V/B value was used to reflect the level of VIP mRNA expression in each tissue. Results The V/B value of intestinal type gastric adenocarcinoma tissue was significantly higher than that of dysplasia gastric mucosa and normal gastric antral mucosa. The V/B values were 1.452 2± 0 .32 82, 0.962 3± 0. 2 2 5 0, 0 .951 3± 0 .2 66 9, the difference was very significant (P < 0.01). There was no significant difference in V/B values between normal antral mucosa and dysplasia gastric mucosa (P > 0.05). Conclusion The expression of VIPmRNA in intestinal gastric adenocarcinoma is significantly higher than that in atypical hyperplasia of gastric mucosa. VIP may play an important role in the development of enteric gastric adenocarcinoma.