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[目的]探讨响应面法优化石墨炉原子吸收光谱法直接测定全血中铅。[方法]以0.5%Triton X-100溶液做血液稀释剂,0.05%氧化钯+0.1%硝酸混合溶液为基体改进剂,直接进样测定。在单因素的基础上,采用中心组合设计和响应面分析法优化灰化温度、原子化温度和基体改进剂用量等因素,并在优化条件下测定小鼠全血铅结果。[结果]响应面法优化石墨炉原子吸收谱法直接测定小鼠全血铅的最优测定条件为灰化温度760.85℃,原子化温度2 486.80℃,基体改进剂加入量0.97 mL。在已优化的条件下测定铅的检出限为0.204μg/L,相对标准差(n=6)为1.76%。染毒小鼠的全血铅含量为82.1μg/L,加标回收率为96.5%~98.4%。[结论]响应面法优化石墨炉原子吸收光谱法不需消化和离心,直接进样,简便快速和准确,适用于染毒小鼠全血铅的测定。
[Objective] The research aimed to discuss the direct determination of lead in whole blood by graphite furnace atomic absorption spectrometry with response surface methodology. [Method] With 0.5% Triton X-100 solution as diluent and 0.05% palladium oxide + 0.1% nitric acid as matrix modifier, the sample was directly injected into the sample. On the basis of single factor, central composite design and response surface analysis were used to optimize factors such as ashing temperature, atomization temperature and dosage of matrix modifier, and the determination of lead in whole blood of mice under optimized conditions. [Result] The optimal determination conditions of direct determination of lead in whole blood of mice by GFAAS by response surface methodology were the ashing temperature 760.85 ℃, the atomization temperature 2486.80 ℃ and the amount of matrix modifier 0.97 mL. The detection limit of Pb was 0.204μg / L under the optimal conditions, and the relative standard deviation (n = 6) was 1.76%. The lead content in the whole blood of the infected mice was 82.1μg / L, and the recoveries were 96.5% ~ 98.4%. [Conclusion] Response surface methodology (GFAAS) was a simple, rapid, accurate and accurate method for determination of lead in whole blood without exposure to digestion and centrifugation.