目的：研究磁性微球与单克隆抗体结合的条件，以构建具有良好性能的免疫磁性微球。方法：以同位素标记的单克隆抗体和微球的结合率为指标。用均匀设计法研究影响免疫磁性微球构建的因素。结果：ｐＨ值、抗体加入量和反应时间是影响免疫磁性微球构建的主要因素。结论：通过控制条件，可以构建具有良好性能的免疫微球。 Objective: To study the conditions of binding of magnetic microspheres with monoclonal antibodies to construct immunomagnetic microspheres with good properties. Methods: The binding rate of isotope labeled monoclonal antibody to microspheres was used as an index. Uniform design method to study the factors that affect the construction of immunomagnetic microspheres. Results: pH, antibody loading and reaction time were the main factors influencing the construction of immunomagnetic microspheres. Conclusion: By controlling the conditions, we can construct immunobeads with good performance.