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目的 了解慢性乙型肝炎病毒 (HBV)感染者外周血中辅助性T细胞 (Th)内干扰素 γ(IFN γ)、白细胞介素 4 (IL 4 )和转化生长因子 β(TGF β)的表达情况 ,以测定Th1/Th2 /Th3细胞的百分数 ,探明Th细胞各极化群体在慢性HBV感染中的作用。方法 常规分离外周血单个核细胞 (PBMC) ,在佛波脂 (PMA)、钙离子导入剂伊屋诺霉素Ionomycin、细胞内转运阻断剂莫能星Monensin的刺激下 ,采用流式细胞分析术 (FACS)对慢性HBV感染者外周血单个Th细胞内IL 4、IFN γ和TGF β的表达进行分析。结果 正常对照组 ,2 3% - 18 6 %的CD4 + T细胞为Th1细胞 ,0 9% - 9 2 %为Th2细胞 ;0 7% - 7 1%的细胞仅表达TGF β ,为Th3细胞。在慢性HBV感染者外周血单个CD4 + T细胞中 ,以Th0细胞居多 ;而Th1细胞则随着慢性乙型肝炎肝脏炎症活动的加剧而明显增多 ,在炎症活动明显的慢性乙型肝炎 ,其Th1百分数明显高于炎症活动呈静止状态的慢性乙型肝炎 ;Th2细胞在慢性HBV感染的不同阶段则较为恒定 (P >0 0 5 ) ,但明显高于对照组 (P<0 0 5 )。Th3细胞的百分数则随着肝组织炎症活动的加剧 ,其比例也明显减少 (P <0 0 5 ) ,在无症状携带者(AsC)组 ,Th3细胞的百分数远高于慢性乙型肝炎组及对照组 (P <0 0 5 )。Th3细胞重叠于Th2细胞之中。结
Objective To investigate the expression of interferon γ (IFN γ), interleukin 4 (IL 4) and transforming growth factor β (TGF β) in peripheral blood of patients with chronic hepatitis B virus (HBV) infection. To determine the percentage of Th1 / Th2 / Th3 cells, the role of each polarized population of Th cells in chronic HBV infection was explored. Methods Peripheral blood mononuclear cells (PBMCs) were isolated routinely and stimulated by PMA and ionomycin ionomycin, Monensin, an intracellular transporter blocker, and analyzed by flow cytometry (FACS) to analyze the expression of IL 4, IFN γ and TGF β in peripheral blood mononuclear cells of patients with chronic HBV infection. Results In the normal control group, 23% -186% of CD4 + T cells were Th1 cells and 0 9% -92% were Th2 cells; while 0 7% -7% of cells expressed only TGFβ, which was Th3 cells. In chronic HBV infected peripheral blood mononuclear CD4 T cells, the majority of Th0 cells; Th1 cells with chronic hepatitis B liver inflammatory activity increased significantly increased in chronic inflammation of chronic hepatitis B, the Th1 The percentages were significantly higher than that of chronic hepatitis B with inflammatory activity. Th2 cells were more constant at different stages of chronic HBV infection (P> 0.05), but significantly higher than those of the control group (P <0.05). The percentage of Th3 cells was significantly decreased with the increase of inflammatory activity in liver tissue (P <0.05). The percentage of Th3 cells in asymptomatic carriers (AsC) group was significantly higher than that in chronic hepatitis B group Control group (P <0 05). Th3 cells overlap in Th2 cells. Knot