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目的:观察三叶青藤正丁醇部位(nbuIR)对胶原性关节炎小鼠关节病变及血清炎症因子的抑制作用。方法:用DBA/1小鼠建立胶原性关节炎(CIA)动物模型,将造模成功小鼠随机分为模型对照组、甲氨蝶呤组、nbuIR低、中、高剂量组,另设正常对照组。灌胃给药,给药期间每周进行一次关节炎指数评分。干预结束后,ELISA法检测各组小鼠血清IL-23和IL-17水平,H-E染色观察各组小鼠踝关节组织病理学改变。结果:与模型对照组比较,nbuIR高剂量组血清IL-23和IL-17水平明显降低(P<0.01),滑膜细胞增生与炎症细胞浸润程度明显减轻。结论:nbuIR能改善CIA小鼠局部关节肿胀,下调血清IL-23和IL-17水平,减少滑膜炎症程度,其机制可能与调节IL-23/IL-17轴有关。
Objective: To observe the inhibitory effect of nbuIR on the joint pathological changes and serum inflammatory cytokines in collagen induced arthritis mice. Methods: Collagen-induced arthritis (CIA) animal model was established by DBA / 1 mice. The successful model mice were randomly divided into model control group, methotrexate group, nbuIR low, medium and high dose groups, Control group. Gavage administration, arthritis index score was administered once a week during the administration. After the intervention, the levels of IL-23 and IL-17 in serum of mice in each group were detected by ELISA, and the pathological changes of ankle joint in mice were observed by H-E staining. Results: Compared with the model control group, the serum levels of IL-23 and IL-17 in nbuIR high-dose group were significantly decreased (P <0.01), and the synovial cell proliferation and infiltration of inflammatory cells were significantly reduced. Conclusion: nbuIR can improve the local joint swelling, reduce the serum IL-23 and IL-17 levels and reduce the synovial inflammation in CIA mice. The mechanism may be related to the regulation of IL-23 / IL-17 axis.