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目的研究新疆紫草提取物AEJ-I、AEJ-Ⅱ对小鼠急性四氯化碳(CC l4)性肝损伤的保护作用。方法腹腔注射0.1%CC l4橄榄油溶液制备小鼠急性化学性肝损伤模型,以肝脏系数、脾脏系数,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的水平和肝脏病理组织学变化为观测指标,观察AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤的影响。结果AEJ-I、AEJ-Ⅱ各剂量组均可显著降低小鼠血清ALT,AST活性(P<0.01);AEJ-I高剂量组能使肝组织中SOD、GSH-Px活性升高、MDA含量下降(P<0.05);AEJ-I低剂量组可以升高肝组织GSH-Px活性(P<0.05),而对肝组织其它指标均无明显影响(P>0.05);AEJ-Ⅱ高剂量组能降低MDA含量(P<0.05),而对肝组织其它指标AEJ-Ⅱ各剂量组影响均不明显(P>0.05);AEJ-I、AEJ-Ⅱ均可不同程度地改善肝脏病理组织性损伤。结论AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤具有保护作用。
Objective To investigate the protective effect of AeJ-I and AEJ-II from Xinjiang comfrey on acute liver injury induced by carbon tetrachloride in mice. METHODS: Acute liver injury model in mice was prepared by intraperitoneal injection of 0.1% CC l4 olive oil solution. The liver coefficient, spleen coefficient, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and histopathological changes in the liver were used as observations to observe AEJ-I and AEJ-II. Effect of acute CC l4 liver injury in mice. Results AEJ-I and AEJ-II all significantly reduced the serum ALT and AST activity (P<0.01); AEJ-I high-dose group could increase the activity of SOD and GSH-Px in liver tissue and MDA content. Decreased (P<0.05); AEJ-I low-dose group can increase the activity of GSH-Px in liver tissue (P<0.05), but has no significant effect on other indicators of liver tissue (P>0.05); AEJ-II high-dose group The level of MDA could be decreased (P<0.05), while the effect of other AEJ-II doses on liver tissue was not significant (P>0.05). AEJ-I and AEJ-II could improve the pathological tissue damage of liver to varying degrees. . Conclusion AEJ-I and AEJ-II have protective effects on acute CC l4 liver injury in mice.