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目的:探讨分析Twist基因在人胃组织、癌旁组织、胃癌转移灶组织中的表达和临床意义。方法:选取我院2013年4月至2014年11月期间接收治疗的52例胃癌患者作为研究分析对象,运用免疫组化法检测Twist蛋白状况,运用PCR检测Twist mRNA状况,并将所有患者的临床资料进行回顾分析,并总结。结果:根据检查结果可将胃癌肿瘤患者按照浸润深度和转移状况进行分组,通过Twist检测,Twist蛋白在胃癌组织中的表达明显高于在癌旁组织中的表达,且数据具有统计学意义(P<0.05)。结论:Twist基因在人胃癌中的表达状况与癌旁组织相比均由上调现象,这可能示意着在胃癌形成中有Twist基因的参与,因此控制Twist基因对控制和预防胃癌疾病应有良好的作用,且Twist基因最胃癌转移中有较大作用,因此对Twist基因进行干扰,对改善胃癌患者预后状况有一定的意义。
Objective: To investigate the expression and clinical significance of Twist gene in human gastric tissues, paracancerous tissues and metastatic gastric cancer tissues. Methods: Fifty-two gastric cancer patients receiving treatment from April 2013 to November 2014 in our hospital were selected as research objects. Twist protein expression was detected by immunohistochemistry. The status of Twist mRNA was detected by PCR. The clinical data of all patients Data review and analysis, and concluded. Results: According to the results of examination, the gastric cancer patients were grouped according to the depth of invasion and metastasis. The expression of Twist protein in gastric cancer tissues was significantly higher than that in paracancerous tissues by Twist test and the data was statistically significant (P <0.05). CONCLUSIONS: The expression of Twist gene in human gastric cancer is up-regulated compared with that in paracancerous tissues, which may indicate the involvement of Twist gene in the formation of gastric cancer. Therefore, controlling Twist gene should have a good effect on the control and prevention of gastric cancer Role, and Twist gene most gastric cancer metastasis has a greater role, so the Twist gene interference, to improve the prognosis of gastric cancer patients have a certain significance.