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目的探讨miR-200b介导鼻咽癌放疗抵抗,揭示miR-200b作为抑癌基因作用。方法 Real-time PCR检测miR-200b在鼻咽癌细胞系中的含量;将miR-200b模拟物转染CNE-2,miR-200b抑制剂转染CNE-2R,采用MTS检测其对CNE-2细胞生长的影响。结果 miR-200b在CNE-2R细胞中表达下调。特异性miR-200b模拟物具有逆转CNE-2R细胞的辐射抗性作用,而转染miR-200b抑制剂可增强CNE-2细胞的辐射抗性。过表达miR-200b可抑制鼻咽癌细胞的生长。结论 miR-200b参与了鼻咽癌细胞的放射抵抗,miR-200b可能成为一种新的用于合理治疗鼻咽癌的治疗策略。
Objective To investigate the radioresistance of miR-200b mediated nasopharyngeal carcinoma and to reveal the role of miR-200b as a tumor suppressor gene. Methods Real-time PCR was used to detect the content of miR-200b in nasopharyngeal carcinoma cell lines. The miR-200b mimics were transfected into CNE-2 and the miR-200b inhibitor was transfected into CNE-2R. Effect of cell growth. Results miR-200b was down-regulated in CNE-2R cells. Specific miR-200b mimics reversed the radioresistance of CNE-2R cells, while transfection of miR-200b inhibitors enhanced the radiation resistance of CNE-2 cells. Overexpression of miR-200b can inhibit the growth of nasopharyngeal carcinoma cells. Conclusion miR-200b is involved in the radioresistance of nasopharyngeal carcinoma cells. MiR-200b may be a new therapeutic strategy for the rational treatment of nasopharyngeal carcinoma.