从胰岛素的受体和受体后作用机制来探讨蚕丝水解物 (silk hydrates,SH)调节糖代谢的机理。选用若干只雄性成年 Wistar大鼠随机分为 2组 :一组经尾静脉注射小剂量链脲佐菌素 (STZ,30 m g/kg BW) 3周后 ,加喂高糖、高脂、高热量饲料。选择具有 型糖尿病特征 ,即糖耐量异常、胰岛素高于或等于对照组的38只大鼠为糖尿病模型组。另一组 36只大鼠不注射 STZ,仅喂普通饲料 ,为对照组。再分别将 2组大鼠各随机分为 2小组 :糠尿病对照组、糖尿病 + SH组 ;正常对照组、正常 + SH组。每日给予 SH 1g/kg BW,观察 12周 ,在实验末每组随机取半数大鼠做骨骼肌胰岛素受体结合实验 ;其余大鼠做葡萄糖摄取实验。结果表明 :1SH能显著改善糖尿病大鼠的糖耐量 ,但对正常大鼠及糖尿病大鼠胰岛素的分泌基本没有影响 ;2给予 SH后 ,糖尿病大鼠骨骼肌细胞膜胰岛素受体对 1 2 5 I-胰岛素的特异结合率没有明显提高 ,仍低于正常大鼠 ;仅低亲和力位点上的受体亲和力常数显著增加 ;3给予 SH后 ,糖尿病大鼠的骨骼肌细胞对葡萄糖的摄取显著增加 ;其自由摄食状态下的肝糖原、骨骼肌糖原的含量显著增加 ;4给予 SH后 ,糖尿病大鼠部分血脂紊乱得以改善。结果提示 SH主要是通过胰岛素的受体后作用机制来改善 型糖尿病模型大鼠血糖代谢的紊乱 The mechanisms by which silk hydrates (SH) regulate glucose metabolism are explored from the mechanism of insulin receptor and receptor rear effects. A number of male adult Wistar rats were randomly divided into two groups: one group was injected with STZ (30 mg / kg BW) through the tail vein for 3 weeks and then fed with high glucose, high fat and high calorie feed. Thirty-eight diabetic patients with diabetes mellitus, with impaired glucose tolerance, insulin higher than or equal to the control group, were selected as the model group. Another group of 36 rats without STZ injection, only fed the normal feed, the control group. The rats in each group were randomly divided into 2 groups: control group of diabetes mellitus and diabetes + SH group; normal control group and normal + SH group. SH 1g / kg BW was given daily for 12 weeks. At the end of the experiment, half of rats in each group were randomly selected for insulin receptor binding assay. The remaining rats were subjected to glucose uptake test. The results showed that: 1SH can significantly improve glucose tolerance in diabetic rats, but insulin secretion in normal rats and diabetic rats had no effect; 2 after SH administration, the insulin receptor of skeletal muscle cells of diabetic rats had no effect on the insulin secretion of 125 I- Insulin specific binding rate did not increase significantly, still lower than normal rats; only low affinity affinity receptor affinity sites significantly increased; 3 after SH administration, diabetic rats skeletal muscle cells uptake of glucose significantly increased; its Liver glycogen and skeletal muscle glycogen in free feeding state significantly increased. 4 After SH administration, some dyslipidemia in diabetic rats was improved. The results suggest that SH mainly improves the disorder of blood glucose metabolism in rats with type 2 diabetes through the mechanism of insulin’s post-receptor action