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以三倍体樱桃矮化砧木‘吉塞拉6号’(Prunusceransus×P.canescens)的离体叶片为外植体,采用秋水仙素诱导处理再生出六倍体植株。将外植体首先在加有秋水仙素(50mg·L-1)、生长素(IBA0·5mg·L-1)和细胞分裂素(BA5·0mg·L-1)的改良WPM液体培养基中培养5d,再转移到不含秋水仙素(其它成分相同)的固体培养基上继续培养56d,再生出形态变异明显的新梢。采用流式细胞仪鉴定染色体倍性,确定其为六倍体新梢。六倍体植株与三倍体的‘吉塞拉6号’植株形态学上有明显差异。六倍体的试管苗已在大田移栽成活,并已成功高接在甜樱桃大树上。
The in vitro leaves of triploid cherry dwarf rootstock ’Prunus ceransus × P. canescens’ were used as explants, and hexaploid plants were regenerated by colchicine induction. The explants were first cultured in modified WPM liquid medium supplemented with colchicine (50 mg · L -1), auxin (IBA 0.5 mg · L -1) and cytokinin (BA 5.0 mg · L -1) Cultured for 5 days, and then transferred to colchicine (other components of the same) solid medium for 56 days to regenerate the obvious morphological changes of the shoots. Chromosome ploidy was identified by flow cytometry and identified as hexaploid shoots. The morphological differences between hexaploid plants and triploid ’Gisela 6’ plants were significant. Hexaploid test tube seedlings have been transplanted in the field survival, and has been successfully high in the sweet cherry tree.