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目的探讨可诱导共刺激分子(Inducible costimulator,ICOS)信号对感染日本血吸虫小鼠的CD154/CD40表达及免疫病理的影响。方法建立ICOS转基因(ICOS transgenic,ICOS-Tg)小鼠及野生型FVB/NJ小鼠日本血吸虫病模型,应用流式细胞术、免疫组化技术分别检测感染前后的小鼠脾淋巴细胞与肝脏虫卵肉芽肿周围炎性浸润细胞CD154、CD40表达水平。应用HE染色法观察小鼠肝脏虫卵肉芽肿病变动态变化。结果与野生型FVB/NJ小鼠相比,ICOS-Tg小鼠脾淋巴细胞CD154、CD40表达水平升高,在感染后12、16周差异有统计学意义(P均<0.05);ICOS-Tg小鼠肝脏虫卵肉芽肿炎性浸润细胞CD154、CD40表达亦显著升高,在感染后7、12、16、20周差异有统计学意义(P均<0.05)。且ICOS-Tg小鼠肝脏虫卵肉芽肿体积显著大于野生型小鼠(P<0.01或0.05)。结论在日本血吸虫感染中,ICOS信号对CD154/CD40表达具有一定的调控作用,在免疫病理中起重要调控作用。
Objective To investigate the effect of inducible Inducible costimulator (ICOS) signal on CD154 / CD40 expression and immunopathology in mice infected with Schistosoma japonicum. Methods ICOS transgenic mice (ICOS-Tg) and wild-type FVB / NJ mice were established. The spleen lymphocytes and liver worms before and after infection were detected by flow cytometry and immunohistochemistry. Egg granuloma around inflammatory infiltration of CD154, CD40 expression levels. HE staining was used to observe the dynamic changes of liver granuloma in mice. Results Compared with wild-type FVB / NJ mice, the expression of CD154 and CD40 in splenic lymphocytes of ICOS-Tg mice increased at 12 and 16 weeks after infection (all P <0.05). ICOS-Tg The expression of CD154 and CD40 in inflammatory granuloma of mouse liver granulomas was also significantly increased. There was a significant difference at 7, 12, 16 and 20 weeks after infection (all P <0.05). The volume of liver granuloma in ICOS-Tg mice was significantly larger than that of wild-type mice (P <0.01 or 0.05). Conclusion ICOS signal plays a regulatory role in the expression of CD154 / CD40 in Schistosoma japonicum infection and plays an important regulatory role in immunopathology.