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目的探讨麻黄素对仔鼠肝组织结构、Bax蛋白的表达和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力、丙二醛(MDA)含量及血清中谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力的影响。方法对48只仔鼠采用递增剂量腹腔连续注射麻黄素溶液(2.0g/L、3.0g/L和4.0g/L)和等量的生理盐水,分别于注射5d、10d和15d称量检测仔鼠肝重的变化,用比色法测定仔鼠肝组织中SOD、CAT活性、MDA含量及血清中GOT和GPT活性的变化,用光学显微镜观察肝脏组织结构的变化,用免疫组织化学法检测肝组织中Bax蛋白表达的变化。结果注射麻黄素5d、10d、15d仔鼠的肝重低于对照组;随着给药时间的延长和药剂量的增加,SOD和CAT的活性先升高后降低,实验组仔鼠5d时SOD、CAT的活性高于对照组,差异显著(P<0.05),10d、15d时SOD、CAT的活性低于对照组,差异显著或极显著(P<0.05或P<0.01);MDA含量则先降低后升高,实验组仔鼠5d时MDA含量低于对照组,差异显著(P<0.05),10d、15d时MDA含量高于对照组,差异极显著(P<0.01)。注射麻黄素后仔鼠血清内GOT和GPT活性高于对照组,差异显著或极显著(P<0.05或P<0.01)。注射麻黄素后仔鼠肝脏有不同程度的损伤,肝板萎缩,肝血窦扩张,细胞界限模糊,内皮细胞脱落;Bax蛋白阳性表达数高于对照组,差异显著或极显著(P<0.05或P<0.01)。结论麻黄素影响发育期仔鼠肝脏的组织结构,这可能与肝组织中抗氧化物酶活性降低和MDA含量升高有关。
Objective To investigate the effects of ephedrine on the liver tissue structure, the expression of Bax and the activities of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and serum aspartate aminotransferase (GOT) Effects of alanine aminotransferase (GPT) activity. Methods Forty-eight offspring rats were injected intraperitoneally with ephedrine (2.0g / L, 3.0g / L and 4.0g / L) and normal saline, respectively. Changes in liver weight and liver weight were measured by colorimetric method. The activity of SOD and CAT, the content of MDA and the activity of GOT and GPT in the liver were detected by colorimetric method. The changes of liver tissue structure were observed with light microscope. Changes of Bax protein expression in tissues. Results The liver weights of ephedrine 5d, 10d and 15d were lower than that of the control group. The activity of SOD and CAT increased first and then decreased with the prolongation of administration time and dose of ephedrine, (P <0.05 or P <0.05). The activities of SOD and CAT in 10d and 15d were lower than those in control group (P <0.05 or P <0.01) (P <0.05). The content of MDA in the experimental group was significantly higher than that in the control group at 5d (P <0.01). The levels of GOT and GPT in serum of offspring rats after injection of ephedrine were significantly higher than those of the control group (P <0.05 or P <0.01). After injecting ephedrine, the liver of the offspring had different degrees of injury, hepatic plate atrophy, hepatic sinusoid dilation, fuzzy cell boundaries and endothelial cell loss; the positive expression of Bax protein was higher than that of the control group (P <0.05 or P <0.01). Conclusion Ephedrine affects the liver tissue of the offspring in development stage, which may be related to the decrease of antioxidant enzyme activity and the increase of MDA content in liver tissue.