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灵芝１～１００μｇ／ｍｌ对Ｌ９２９细胞的生长无直接影响。在加灵芝与不加灵芝的条件下观察重组肿瘤坏死因子（ｒＴＮＦ）对Ｌ９２９细胞的杀伤作用证明灵芝不影响ｒＴＮＦ的杀伤肿瘤活性。但灵芝０．０１～１０μｇ／ｍｌ在试管内促进小鼠腹腔巨噬细胞产生ＴＮＦ。巨噬细胞在试管内经灵芝预培养８ｈ后，灵芝仍可增加巨噬细胞在ＬＰＳ诱导下ＴＮＦ的产生。此外，整体研究证明口服灵芝１００～３００ｍｇ／ｋｇｑｄ×１４可以增高由ＢＣＧ启动及内毒素诱导的小鼠血清ＴＮＦ水平
Ganoderma lucidum 1 ~ 100μg / ml has no direct effect on the growth of L929 cells. To observe the killing effect of rTNF on L929 cells with or without Ganoderma lucidum proved that Ganoderma lucidum does not affect the antitumor activity of rTNF. However, Ganoderma lucidum 0.01 ~ 10μg / ml in vitro promote mouse peritoneal macrophages to produce TNF. After macrophages were preincubated with Ganoderma lucidum in vitro for 8 h, Ganoderma lucidum could still increase the production of TNF by LPS-induced macrophages. In addition, the overall study showed that 100 ~ 300mg / kgqd × 14 oral Ganoderma lucidum can be increased by the BCG promoter and endotoxin-induced serum TNF levels in mice